Department of Cardiovascular Surgery, Gazi University Medical Faculty, 06570 Ankara, Turkey.
Department of Cardiovascular Surgery, Sivas Numune Hospital, 58380 Sivas, Turkey.
Medicina (Kaunas). 2024 May 13;60(5):804. doi: 10.3390/medicina60050804.
: Lower limb skeletal muscle ischemia-reperfusion (IR) injury is associated with increased morbidity and mortality, and it is common in several clinical situations such as aortic aneurysms repairment, peripheral arterial surgery, vascular injury repairment, and shock. Although it is generally accepted that oxidative stress mediators have a significant role in IR injury, its precise mechanism is still unknown. Anecdotally, it is sustained not only by structural and functional changes in the organ it affects but also by damage to distant organs. The purpose of this report is to illustrate the effect of proanthocyanidin on IR injury. : In our study, 18 male Wistar albino rats were used. The subjects were divided into three groups containing six mice each (control, C; ischemia-reperfusion, IR; ischemia-reperfusion and proanthocyanidin; IR-PRO). Intraperitoneal proanthocyanidin was given to the IR and proanthocyanidin groups 30 min before laparotomy, and 1 h ischemia led to these two groups. After one hour, reperfusion started. Muscle atrophy-hypertrophy, muscle degeneration-congestion, fragmentation-hyalinization, muscle oval-central nucleus ratio, leukocyte cell infiltration, catalase enzyme activity, and TBARS were all examined in lower-limb muscle samples after one hour of reperfusion. : When skeletal muscle samples were evaluated histopathologically, it was discovered that muscle atrophy-hypertrophy, muscle degeneration-congestion, fragmentation-hyalinization, and leukocyte cell infiltration with oval-central nucleus standardization were significantly higher in the IR group than in the C and IR-P groups. Oval-central nucleus standardization was significantly higher in the IR and IR-PRO groups than in the control group. TBARS levels were significantly higher in the IR group than in the control and IR-PRO groups, while catalase enzyme activity was found to be significantly lower in the IR group than in the control and IR-PRO groups. : As a consequence of our research, we discovered that proanthocyanidins administered before IR have a protective impact on skeletal muscle in rats. Further research in this area is required.
下肢骨骼肌缺血再灌注（IR）损伤与发病率和死亡率增加有关，它常见于几种临床情况，如主动脉瘤修复、外周动脉手术、血管损伤修复和休克。尽管普遍认为氧化应激介质在 IR 损伤中具有重要作用，但确切的机制仍不清楚。据推测，它不仅受到受影响器官的结构和功能变化的影响，还受到远处器官损伤的影响。本报告的目的是说明原花青素对 IR 损伤的影响。
在我们的研究中，使用了 18 只雄性 Wistar 白化大鼠。将这些对象分为三组，每组包含六只老鼠（对照组 C；缺血再灌注组 IR；缺血再灌注和原花青素组 IR-PRO）。在剖腹术前 30 分钟，给 IR 和原花青素组腹腔内给予原花青素，导致这两组发生 1 小时的缺血。 1 小时后，开始再灌注。在再灌注 1 小时后，检查下肢肌肉样本中的肌肉萎缩-肥大、肌肉变性-充血、碎裂-玻璃样变、肌肉椭圆形-中央核比值、白细胞细胞浸润、过氧化氢酶酶活性和 TBARS。
当对骨骼肌样本进行组织病理学评估时，发现与 C 和 IR-P 组相比，IR 组的肌肉萎缩-肥大、肌肉变性-充血、碎裂-玻璃样变和白细胞细胞浸润伴椭圆形-中央核标准化显著更高。IR 和 IR-PRO 组的椭圆形-中央核标准化显著高于对照组。IR 组的 TBARS 水平显著高于对照组和 IR-PRO 组，而 IR 组的过氧化氢酶酶活性显著低于对照组和 IR-PRO 组。
由于我们的研究，我们发现 IR 前给予原花青素对大鼠骨骼肌具有保护作用。需要进一步研究这一领域。
