Sizlan Ali, Guven Ahmet, Uysal Bulent, Yanarates Omer, Atim Abdulkadir, Oztas Emin, Cosar Ahmet, Korkmaz Ahmet
Department of Anesthesiology and Reanimation, Gulhane Military Medical Academy, Etlik, Ankara, Turkey.
World J Surg. 2009 Jul;33(7):1384-91. doi: 10.1007/s00268-009-0011-9.
Intestinal ischemia/reperfusion (IR) induces a systemic inflammatory response and releases harmful substances that may affect the function and integrity of distant organs such as lung, liver, and kidney. We conducted this study to find out if proanthocyanidins (PA) has protective effects against mesenteric IR injury and mesenteric IR-induced intestinal and distant organ injury.
Thirty-two Sprague-Dawley rats were divided into four groups: control, control + PA, IR, IR + PA. The IR and IR + PA groups were subjected to mesenteric arterial ischemia for 60 min and reperfusion for 6 h. The Control + PA and IR + PA groups were administered PA (100 mg/kg/day via oral gavage) for 7 days prior to injury insult. We collected ileal and distant organ tissues, such as pulmonary, hepatic, and kidney specimens to measure tissue levels of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx), and nitrite plus nitrate (NO(x)), and we then evaluated histological changes.
In the IR group, significant increases in MDA and NO(x) levels and significant increases in SOD and GPx activities of intestine, liver, kidney, and lung were observed. The MDA and NO(x) levels were significantly lower, as were the SOD and GPx activities in the IR + PA group than that in the IR group. Although the intestine and distant organs damage scores were significantly higher in the IR group, these injuries were prevented by PA in the IR + PA group.
This study demonstrates that PA has a significant effect in the protection of the intestine and the remote organs against mesenteric IR injury.
肠道缺血/再灌注（IR）会引发全身炎症反应并释放有害物质，这些物质可能会影响肺、肝和肾等远处器官的功能和完整性。我们开展这项研究以探究原花青素（PA）是否对肠系膜IR损伤以及肠系膜IR诱导的肠道和远处器官损伤具有保护作用。
将32只Sprague-Dawley大鼠分为四组：对照组、对照+PA组、IR组、IR+PA组。IR组和IR+PA组经历肠系膜动脉缺血60分钟及再灌注6小时。对照+PA组和IR+PA组在损伤刺激前7天经口灌胃给予PA（100mg/kg/天）。我们收集回肠和远处器官组织，如肺、肝和肾标本，以测量丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）以及亚硝酸盐加硝酸盐（NO(x)）的组织水平，然后评估组织学变化。
在IR组中，观察到肠道、肝脏、肾脏和肺的MDA和NO(x)水平显著升高，SOD和GPx活性显著增加。IR+PA组的MDA和NO(x)水平显著更低，SOD和GPx活性也低于IR组。尽管IR组的肠道和远处器官损伤评分显著更高，但PA可预防IR+PA组的这些损伤。
本研究表明，PA对保护肠道和远处器官免受肠系膜IR损伤具有显著作用。
