Versura P, Bonvicini F, Caramazza R, Laschi R
Scan Electron Microsc. 1985(Pt 4):1695-708.
Relatively few morphological studies on human cornea have been performed due to the difficulty in obtaining material. The specimens may be obtained either by superficial scraping-off, limited to the epithelial layer, or from corneal buttons obtained at keratoplasty. Conjunctiva, on the other hand, may be obtained by painless biopsy. We have studied the three-dimensional ultrastructure of the normal cornea and conjunctiva and in the course of various diseases where the application of scanning electron microscopy (SEM) seemed to be useful. In the cornea, we studied Fuchs' endo-epithelial dystrophy. In the conjunctiva, we analysed the ultrastructural characteristics of adenovirus follicular conjunctivitis, hay fever conjunctivitis and keratoconjunctivitis sicca. We also considered the problem of contact lens wearing, studying by SEM both the conjunctival tissue and the discarded lenses. On the basis of our results we conclude that SEM supplies useful information which integrates that obtained by biomicroscopy, used by clinicians to interpret the lesions at microscopic level. It is, however, indispensable to carry out, in addition to SEM, an examination on sections to completely investigate the disease.
由于获取材料困难，针对人类角膜的形态学研究相对较少。标本可以通过仅限于上皮层的表面刮除获得，或者从角膜移植术中获取的角膜片获得。另一方面，结膜可以通过无痛活检获得。我们研究了正常角膜和结膜的三维超微结构，以及在各种疾病过程中扫描电子显微镜（SEM）似乎有用的情况。在角膜方面，我们研究了富克斯内皮性角膜营养不良。在结膜方面，我们分析了腺病毒滤泡性结膜炎、花粉症结膜炎和干燥性角结膜炎的超微结构特征。我们还考虑了佩戴隐形眼镜的问题，通过扫描电子显微镜研究结膜组织和丢弃的镜片。根据我们的结果，我们得出结论，扫描电子显微镜提供了有用的信息，这些信息整合了临床医生用于在微观层面解释病变的生物显微镜所获得的信息。然而，除了扫描电子显微镜之外，进行切片检查以全面研究疾病是必不可少的。
