编码甲型禽流感病毒H5N8修饰血凝素三聚体的DNA疫苗可保护小鼠免受病毒攻击。
DNA Vaccine Encoding a Modified Hemagglutinin Trimer of Avian Influenza A Virus H5N8 Protects Mice from Viral Challenge.
作者信息
Litvinova Victoria R, Rudometov Andrey P, Rudometova Nadezhda B, Kisakov Denis N, Borgoyakova Mariya B, Kisakova Lyubov A, Starostina Ekaterina V, Fando Anastasia A, Yakovlev Vladimir A, Tigeeva Elena V, Ivanova Ksenia I, Gudymo Andrei S, Ilyicheva Tatiana N, Marchenko Vasiliy Yu, Sergeev Artemiy A, Ilyichev Alexander A, Karpenko Larisa I
机构信息
Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
出版信息
Vaccines (Basel). 2024 May 14;12(5):538. doi: 10.3390/vaccines12050538.
The development of a safe and effective vaccine against avian influenza A virus (AIV) H5N8 is relevant due to the widespread distribution of this virus in the bird population and the existing potential risk of human infection, which can lead to significant public health concerns. Here, we developed an experimental pVAX-H5 DNA vaccine encoding a modified trimer of AIV H5N8 hemagglutinin. Immunization of BALB/c mice with pVAX-H5 using jet injection elicited high titer antibody response (the average titer in ELISA was 1 × 10), and generated a high level of neutralizing antibodies against H5N8 and T-cell response, as determined by ELISpot analysis. Both liquid and lyophilized forms of pVAX-H5 DNA vaccine provided 100% protection of immunized mice against lethal challenge with influenza A virus A/turkey/Stavropol/320-01/2020 (H5N8). The results obtained indicate that pVAX-H5 has good opportunities as a vaccine candidate against the influenza A virus (H5N8).
鉴于甲型禽流感病毒（AIV）H5N8在鸟类群体中的广泛传播以及存在的人类感染潜在风险，这可能引发重大公共卫生问题，因此研发一种安全有效的H5N8疫苗具有重要意义。在此，我们研发了一种实验性的pVAX - H5 DNA疫苗，其编码经修饰的AIV H5N8血凝素三聚体。使用喷射注射法用pVAX - H5免疫BALB/c小鼠引发了高滴度抗体反应（ELISA中的平均滴度为1×10），并产生了高水平的针对H5N8的中和抗体以及T细胞反应，这通过ELISpot分析得以确定。pVAX - H5 DNA疫苗的液体和冻干形式均为免疫小鼠提供了100%的保护，使其免受甲型流感病毒A/土耳其/斯塔夫罗波尔/320 - 01/2020（H5N8）的致死性攻击。所获得的结果表明，pVAX - H5作为一种针对甲型流感病毒（H5N8）的候选疫苗具有良好的前景。
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