Department of Electrical Engineering, Faculty of Engineering, Rajamangala University of Technology Thanyaburi, Pathum Thani 12110, Thailand.
Department of Electrical Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok 10330, Thailand.
Sensors (Basel). 2024 May 17;24(10):3186. doi: 10.3390/s24103186.
Malaria is a disease that affects millions of people worldwide, particularly in developing countries. The development of accurate and efficient methods for the detection of malaria-infected cells is crucial for effective disease management and control. This paper presents the electrical impedance spectroscopy (EIS) of normal and malaria-infected red blood cells. An EIS microfluidic device, comprising a microchannel and a pair of coplanar electrodes, was fabricated for single-cell measurements in a continuous manner. Based on the EIS results, the aim of this work is to discriminate -infected red blood cells from the normal ones. Different from typical impedance spectroscopy, our measurement was performed for the cells in a low-conductivity medium in a frequency range between 50 kHz and 800 kHz. Numerical simulation was utilized to study the suitability parameters of the microchannel and electrodes for the EIS experiment over the measurement frequencies. The measurement results have shown that by using the low-conductivity medium, we could focus on the change in the conductance caused by the presence of a cell in the sensing electrode gap. The results indicated a distinct frequency spectrum of the conductance between the normal and infected red blood cells, which can be further used for the detection of the disease.
疟疾是一种影响全球数百万人的疾病，特别是在发展中国家。开发准确高效的疟疾感染细胞检测方法对于有效疾病管理和控制至关重要。本文介绍了正常和疟疾感染的红细胞的电阻抗谱（EIS）。为了进行连续的单细胞测量，制作了一个包含微通道和一对共面电极的 EIS 微流控装置。基于 EIS 结果，本工作旨在区分正常红细胞和感染红细胞。与典型的阻抗谱不同，我们的测量是在频率范围为 50 kHz 至 800 kHz 的低电导率介质中对细胞进行的。数值模拟用于研究微通道和电极在测量频率下对 EIS 实验的适用性参数。测量结果表明，通过使用低电导率介质，我们可以专注于由于传感电极间隙中存在细胞而引起的电导变化。结果表明正常和感染的红细胞之间存在明显的电导频谱差异，可进一步用于疾病检测。
