Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario Monte S. Angelo, Naples, Italy.
Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario Monte S. Angelo, Naples, Italy.
Carbohydr Res. 2024 Jul;541:109148. doi: 10.1016/j.carres.2024.109148. Epub 2024 May 20.
Shewanella vesiculosa HM13 is a Gram-negative bacterium able to produce a large amount of extracellular membrane vesicles. These nanoparticles carry a major protein P49, the loading of which seems to be influenced by the glycans decorating the membrane. Here we report the structural characterization, using chemical analyses and NMR spectroscopy, of the capsular polysaccharides isolated from the nfnB-mutant strain of S. vesiculosa HM13, which is unable to load P49 on the membrane vesicles. In addition to the polysaccharide corona isolated and characterized from the parental strain, the nfnB-mutant strain released another polysaccharide composed of disaccharide repeating units having the following structure. →4)-β-D-Glc-(1 → 3)-β-D-GlcNAc-(1→.
希瓦氏菌属 HM13 是一种革兰氏阴性菌，能够产生大量的细胞外膜泡。这些纳米颗粒携带一种主要的蛋白质 P49，其装载似乎受到修饰膜的聚糖的影响。在这里，我们使用化学分析和 NMR 光谱法报告了从 S. vesiculosa HM13 的 nfnB 突变株中分离的荚膜多糖的结构特征，该突变株不能将 P49 装载到膜泡上。除了从亲本菌株中分离和表征的多糖冠外，nfnB 突变株还释放了另一种由二糖重复单元组成的多糖，其结构如下：→4)-β-D-Glc-(1 → 3)-β-D-GlcNAc-(1→。
