Centre for Advanced Jet Engineering Technology (CaJET), Key Laboratory of High-efficiency and Clean Mechanical Manufacture (Ministry of Education), National Experimental Teaching Demonstration Center for Mechanical Engineering (Shandong University), School of Mechanical Engineering, Shandong University, Jinan 250061, China.
School of Mechanical Engineering, Yanshan University, Qinhuangdao 066004, China.
Biomater Adv. 2024 Jul;161:213895. doi: 10.1016/j.bioadv.2024.213895. Epub 2024 May 21.
Ischemic stroke, a cerebrovascular disease caused by arterial occlusion in the brain, can lead to brain impairment and even death. Stem cell therapies have shown positive advantages to treat ischemic stroke because of their extended time window, but the cell viability is poor when transplanted into the brain directly. Therefore, a new hydrogel GelMA-T was developed by introducing taurine on GelMA to transplant neural stem cells. The GelMA-T displayed the desired photocuring ability, micropore structure, and cytocompatibility. Its compressive modulus was more similar to neural tissue compared to that of GelMA. The GelMA-T could protect SH-SY5Y cells from injury induced by OGD/R. Furthermore, the NE-4C cells showed better proliferation performance in GelMA-T than that in GelMA during both 2D and 3D cultures. All results demonstrate that GelMA-T possesses a neuroprotective effect for ischemia/reperfusion injury against ischemic stroke and plays a positive role in promoting NSC proliferation. The novel hydrogel is anticipated to function as cell vehicles for the transplantation of neural stem cells into the stroke cavity, aiming to treat ischemic stroke.
缺血性脑卒中是一种由大脑动脉阻塞引起的脑血管疾病，可导致脑损伤甚至死亡。干细胞疗法因其治疗时间窗口延长而显示出治疗缺血性脑卒中的积极优势，但直接移植到大脑中时细胞活力较差。因此，通过在 GelMA 上引入牛磺酸开发了一种新型水凝胶 GelMA-T 来移植神经干细胞。GelMA-T 显示出所需的光固化能力、微孔结构和细胞相容性。与 GelMA 相比，其压缩模量更接近神经组织。GelMA-T 可以保护 SH-SY5Y 细胞免受 OGD/R 诱导的损伤。此外，在 2D 和 3D 培养中，NE-4C 细胞在 GelMA-T 中的增殖性能均优于 GelMA。所有结果均表明，GelMA-T 对缺血再灌注损伤具有神经保护作用，可对抗缺血性脑卒中，并在促进 NSC 增殖方面发挥积极作用。这种新型水凝胶有望作为神经干细胞移植到脑卒中腔中的细胞载体，用于治疗缺血性脑卒中。
