大口黑鲈（天堂鱼）的参考基因组。

The reference genome of Macropodus opercularis (the paradise fish).

机构信息

Department of Genetics, ELTE Eötvös Loránd University, Budapest, Hungary.

Translational and Functional Genomics Branch, National Human Genome Research Institute, Bethesda, MD, USA.

出版信息

Sci Data. 2024 May 25;11(1):540. doi: 10.1038/s41597-024-03277-1.

DOI:10.1038/s41597-024-03277-1

PMID:38796485

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11127978/

Abstract

Amongst fishes, zebrafish (Danio rerio) has gained popularity as a model system over most other species and while their value as a model is well documented, their usefulness is limited in certain fields of research such as behavior. By embracing other, less conventional experimental organisms, opportunities arise to gain broader insights into evolution and development, as well as studying behavioral aspects not available in current popular model systems. The anabantoid paradise fish (Macropodus opercularis), an "air-breather" species has a highly complex behavioral repertoire and has been the subject of many ethological investigations but lacks genomic resources. Here we report the reference genome assembly of M. opercularis using long-read sequences at 150-fold coverage. The final assembly consisted of 483,077,705 base pairs (~483 Mb) on 152 contigs. Within the assembled genome we identified and annotated 20,157 protein coding genes and assigned ~90% of them to orthogroups.

摘要

在鱼类中，斑马鱼（Danio rerio）作为一种模型系统比其他大多数鱼类更受欢迎。虽然它们作为模型的价值已经得到充分证明，但在某些研究领域，如行为学，它们的用途有限。通过采用其他不那么传统的实验生物，我们有机会更全面地了解进化和发育，并研究当前流行的模型系统中无法研究的行为学方面。攀鲈科的天堂鱼（Macropodus opercularis）是一种“空气呼吸”物种，其行为学表现非常复杂，已经成为许多行为学研究的对象，但缺乏基因组资源。在这里，我们使用 150 倍覆盖率的长读序列报告了 M. opercularis 的参考基因组组装。最终组装由 152 个 contigs 组成，包含 483,077,705 个碱基对（约 483Mb）。在组装的基因组中，我们鉴定并注释了 20,157 个蛋白质编码基因，并将它们中的约 90%分配到了直系同源基因群中。