Department of Orthopedics and Traumatology, Private Buhara Hospital, Erzurum, Turkey.
Department of Biochemistry, Faculty of Veterinary Medicine, Atatürk University, Erzurum, Turkey.
Am J Sports Med. 2022 Feb;50(2):486-498. doi: 10.1177/03635465211059821. Epub 2021 Dec 15.
Achilles tendinopathy, seen in athletes and manual labor workers, is an inflammatory condition characterized by chronic tendon pain. Owing to the toxicity that develops in various organs attributed to the use of anti-inflammatory drugs, there is a need for new therapeutic agents.
In the present study, the effects of quercetin (Que), the one that attracted the most attention of researchers studying this group of flavonoids, were investigated against collagenase-induced tendinopathy.
Controlled laboratory study.
A total of 35 Sprague-Dawley rats were used in the study. Tendinopathy was created by injecting a single dose of collagenase (10 μL; 10 mg/mL) into the tendons of rats. Thirty minutes after the injection, Que was administered at doses of 25 or 50 mg/kg. Que administration was carried out for 7 days. Animals underwent a motility test at the end of the study. In addition, markers of oxidative stress, inflammation, apoptosis, and autophagy, as well as the expression levels of matrix metalloproteinases (MMPs 2, 3, 9, and 13), ICAM-1, and STAT3, were measured in tendon tissues with biochemical, molecular, and Western blot techniques.
The results showed that oxidative stress, inflammation, apoptosis, and autophagy were triggered by the injection of collagenase. In addition, MMPs, ICAM-1, and STAT3 were activated to participate in the development of tendinopathy. Que was found to reduce ICAM-1 levels in tendon tissue. Moreover, Que showed antioxidant, anti-inflammatory, antiapoptotic, and antiautophagic effects on tendons against tendinopathy. More important, Que suppressed the expression of MMPs in the tendon tissues.
Que has protective properties against collagenase-induced tendon damage in rats.
We believe that with further study, Que may be shown to be an alternative treatment option for athletes or others who experience tendon injuries.
跟腱病见于运动员和体力劳动者，是一种以慢性跟腱疼痛为特征的炎症性疾病。由于使用抗炎药会导致各种器官中毒，因此需要新的治疗药物。
本研究旨在研究槲皮素（Que）对胶原酶诱导的腱病的作用。研究人员研究这组类黄酮时，Que 引起了最多的关注。
对照实验室研究。
本研究共使用 35 只 Sprague-Dawley 大鼠。通过向大鼠肌腱内单次注射 10 μL（10mg/mL）胶原酶来建立腱病模型。注射后 30 分钟，给予 Que 25 或 50mg/kg 剂量。Que 给药持续 7 天。研究结束时，动物进行运动测试。此外，还通过生化、分子和 Western blot 技术测量肌腱组织中氧化应激、炎症、细胞凋亡和自噬的标志物以及基质金属蛋白酶（MMPs 2、3、9 和 13）、ICAM-1 和 STAT3 的表达水平。
结果表明，胶原酶注射会引发氧化应激、炎症、细胞凋亡和自噬。此外，MMPs、ICAM-1 和 STAT3 被激活参与腱病的发生。Que 被发现可降低肌腱组织中 ICAM-1 的水平。此外，Que 对腱组织具有抗氧化、抗炎、抗凋亡和抗自噬作用，可预防腱病。更重要的是，Que 抑制了肌腱组织中 MMPs 的表达。
Que 对大鼠胶原酶诱导的肌腱损伤具有保护作用。
我们相信，随着进一步的研究，Que 可能被证明是运动员或其他经历肌腱损伤的人的一种替代治疗选择。
