Division of Thoracic and Cardiovascular Surgery, Department of Surgery, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung.
Institute for Translational Research in Biomedicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung.
Cell Transplant. 2024 Jan-Dec;33:9636897241253144. doi: 10.1177/09636897241253144.
This study tested the hypothesis that ITRI Biofilm prevents adhesion of the chest cavity. Combined extracorporeal shock wave (ECSW) + bone marrow-derived autologous endothelial progenitor cell (EPC) therapy was superior to monotherapy for improving heart function (left ventricular ejection fraction [LVEF]) in minipigs with ischemic cardiomyopathy (IC) induced by an ameroid constrictor applied to the mid-left anterior descending artery. The minipigs ( = 30) were equally designed into group 1 (sham-operated control), group 2 (IC), group 3 (IC + EPCs/by directly implanted into the left ventricular [LV] myocardium; 3 [+]/3[-] ITRI Biofilm), group 4 (IC + ECSW; 3 [+]/[3] - ITRI Biofilm), and group 5 (IC + EPCs-ECSW; 3 [+]/[3] - ITRI Biofilm). EPC/ECSW therapy was administered by day 90, and the animals were euthanized, followed by heart harvesting by day 180. studies demonstrated that cell viability/angiogenesis/cell migratory abilities/mitochondrial concentrations were upregulated in EPCs treated with ECSW compared with those in EPCs only (all s < 0.001). The LVEF was highest in group 1/lowest in group 2/significantly higher in group 5 than in groups 3/4 (all s < 0.0001) by day 180, but there was no difference in groups 3/4. The adhesion score was remarkably lower in patients who received ITRI Biofilm treatment than in those who did not (all s <0.01). The protein expressions of oxidative stress (NOX-1/NOX-2/oxidized protein)/apoptotic (mitochondrial-Bax/caspase3/PARP)/fibrotic (TGF-β/Smad3)/DNA/mitochondria-damaged (γ-H2AX/cytosolic-cytochrome-C/p-DRP1), and heart failure/pressure-overload (BNP [brain natriuretic peptide]/β-MHC [beta myosin heavy chain]) biomarkers displayed a contradictory manner of LVEF among the groups (all s < 0.0001). The protein expression of endothelial biomarkers (CD31/vWF)/small-vessel density revealed a similar LVEF within the groups (all s < 0.0001). ITRI Biofilm treatment prevented chest cavity adhesion and was superior in restoring IC-related LV dysfunction when combined with EPC/ECSW therapy compared with EPC/ECSW therapy alone.
这项研究检验了一个假设，即 ITri 生物膜可防止胸腔粘连。在通过应用于左前降支中段的 ameroid 缩窄器诱导缺血性心肌病（IC）的小型猪中，联合体外冲击波（ECSW）+骨髓来源的自体内皮祖细胞（EPC）治疗优于单独的 EPC 治疗，可改善心功能（左心室射血分数[LVEF]）。小型猪（=30）被平均设计为 1 组（假手术对照）、2 组（IC）、3 组（IC+EPCs/直接植入左心室[LV]心肌；3[+]/3[-]ITri 生物膜）、4 组（IC+ECSW；3[+]/3[-]ITri 生物膜）和 5 组（IC+EPC-ECSW；3[+]/3[-]ITri 生物膜）。EPC/ECSW 治疗于第 90 天开始，第 180 天处死动物，然后进行心脏采集。研究表明，与仅 EPC 相比，用 ECSW 处理的 EPC 的细胞活力/血管生成/细胞迁移能力/线粒体浓度上调（均 s<0.001）。第 180 天时，1 组的 LVEF 最高，2 组最低，5 组明显高于 3 组/4 组（均 s<0.0001），但 3 组/4 组之间无差异。接受 ITri 生物膜治疗的患者的粘连评分明显低于未接受治疗的患者（均 s<0.01）。氧化应激（NOX-1/NOX-2/氧化蛋白）/凋亡（线粒体-Bax/caspase3/PARP）/纤维化（TGF-β/Smad3）/DNA/线粒体损伤（γ-H2AX/细胞质-细胞色素-C/p-DRP1）和心力衰竭/压力超负荷（BNP[脑利钠肽]/β-MHC[β 肌球蛋白重链]）生物标志物的蛋白表达在各组之间呈相反的 LVEF 方式（均 s<0.0001）。内皮生物标志物（CD31/vWF）/小血管密度的蛋白表达在各组内显示出相似的 LVEF（均 s<0.0001）。与单独的 EPC/ECSW 治疗相比，ITri 生物膜治疗可预防胸腔粘连，并在与 EPC/ECSW 联合治疗时更有效地恢复与 IC 相关的 LV 功能障碍。
