Chair of Animal Physiology and Immunology, School of Life Sciences, Technical University of Munich, Munich, Germany.
Faculty of Agriculture and Veterinary, Department of Animal Biotechnology, University of Prishtina, Prishtina, Kosovo.
Reprod Domest Anim. 2024 May;59(5):e14624. doi: 10.1111/rda.14624.
The study aimed to assess the local gene expression of adipokine members, namely vaspin, adiponectin, visfatin, resistin and their associated receptors - heat shock 70 protein 5 (HSPA5), adiponectin receptor 1 (AdipoR1) and adiponectin receptor 2 (AdipoR2) - in bovine follicles during the preovulatory period and early corpus luteum development. Follicles were collected before gonadotropin-releasing hormone (GnRH) treatment (0 h) and at 4, 10, 20, 25 and 60 h after GnRH application through transvaginal ovariectomy (n = 5 samples/group). Relative mRNA expression levels were quantified using real-time reverse transcription polymerase chain reaction (RT-qPCR). Vaspin exhibited high mRNA levels immediately 4 h after GnRH application, followed by a significant decrease. Adiponectin mRNA levels were elevated at 25 h after GnRH treatment. AdipoR2 exhibited late-stage upregulation, displaying increased expression at 20, 25 and 60 h following GnRH application. Visfatin showed upregulation at 20 h post-GnRH application. In conclusion, the observed changes in adipokine family members within preovulatory follicles, following experimentally induced ovulation, may constitute crucial components of the local mechanisms regulating final follicle growth and development.
本研究旨在评估在促性腺激素释放激素（GnRH）处理前（0 小时）和 GnRH 应用后 4、10、20、25 和 60 小时通过经阴道卵巢切除术（n=5 个样本/组）收集的牛卵泡中脂肪因子成员（即 vaspin、脂联素、内脂素、抵抗素及其相关受体——热休克蛋白 70 蛋白 5（HSPA5）、脂联素受体 1（AdipoR1）和脂联素受体 2（AdipoR2））的局部基因表达。使用实时逆转录聚合酶链反应（RT-qPCR）定量相对 mRNA 表达水平。在 GnRH 应用后 4 小时，vaspin 表现出高 mRNA 水平，随后显著下降。脂联素 mRNA 水平在 GnRH 处理后 25 小时升高。AdipoR2 在 GnRH 应用后 20、25 和 60 小时表现出晚期上调，表达增加。内脂素在 GnRH 应用后 20 小时上调。总之，在实验诱导排卵后，在促排卵前卵泡中观察到的脂肪因子家族成员的变化，可能构成调节最终卵泡生长和发育的局部机制的重要组成部分。
