Chair of Animal Physiology and Immunology, School of Life Sciences, Technical University of Munich, Munich, Germany.
Department of Animal Biotechnology, Faculty of Agriculture and Veterinary, University of Prishtina, Prishtina, Kosovo.
Reprod Domest Anim. 2024 Sep;59(9):e14722. doi: 10.1111/rda.14722.
The objective of the study was to characterise the expression patterns of the two key components of cortisol action namely HSD11B1 (11-beta-hydroxysteroid dehydrogenase type 1) and NR3C1 (nuclear receptor subfamily 3, group C, member 1, also known as the glucocorticoid receptor) in superovulation induced bovine follicles during the periovulation and subsequent corpus luteum (CL) formation. Bovine ovaries containing preovulatory follicles or CL were timely defined during induced ovulation as follows: 0 h before GnRH (Gonadotropin-releasing hormone) application, and 4, 10, 20, 25 (follicles) and 60 h (early CL) after GnRH. The low mRNA expression of HSD11B1 and NR3C1 in the follicle group before the GnRH application increased significantly in the follicle group 20 h after GnRH and remained high afterward also in the early CL group. In contrast, the high NR3C1 mRNA decreased in follicles 25 h after GnRH (close to ovulation) and significantly increased again after ovulation (early CL). Our results indicated the involvement of HSD11B1 and NR3C1 as the two key components of cortisol action in the local mechanisms coordinating final follicle maturation, ovulation, follicular-luteal transition and CL development in the cow.
本研究旨在描述皮质醇作用的两个关键组成部分在超排卵诱导的牛卵泡中的表达模式，这两个关键组成部分是 11-β-羟类固醇脱氢酶 1（HSD11B1）和核受体亚家族 3、C 组、成员 1（也称为糖皮质激素受体，NR3C1）。在诱导排卵期间，及时将含有预排卵卵泡或黄体（CL）的牛卵巢定义为：在 GnRH（促性腺激素释放激素）应用前 0 小时，以及 GnRH 应用后 4、10、20、25 小时（卵泡）和 60 小时（早期 CL）。在 GnRH 应用前的卵泡组中，HSD11B1 和 NR3C1 的低 mRNA 表达在 GnRH 应用后 20 小时的卵泡组中显著增加，并在随后的早期 CL 组中保持高水平。相比之下，NR3C1 的高 mRNA 在 GnRH 应用后 25 小时的卵泡中减少（接近排卵），并在排卵后（早期 CL）再次显著增加。我们的结果表明，HSD11B1 和 NR3C1 作为皮质醇作用的两个关键组成部分，参与了牛体内协调最终卵泡成熟、排卵、卵泡黄体化和 CL 发育的局部机制。
