Ramatchandirin Balamurugan, Balamurugan Marie Amalie, Desiraju Suneetha, Chung Yerin, MohanKumar Krishnan
Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE, USA.
Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Bio Protoc. 2024 May 20;14(10):e4993. doi: 10.21769/BioProtoc.4993.
Anemia is a common and serious health problem, nearly universally diagnosed in preterm infants, and is associated with increased morbidity and mortality worldwide. Red blood cell (RBC) transfusion is a lifesaving and mainstay therapy; however, it has critical adverse effects. One consequence is necrotizing enterocolitis (NEC), an inflammatory bowel necrosis disease in preterm infants. The murine model of phlebotomy-induced anemia and RBC transfusion-associated NEC enables a detailed study of the molecular mechanisms underlying these morbidities and the evaluation of potential new therapeutic strategies. This protocol describes a detailed procedure for obtaining murine pups with phlebotomy-induced anemia and delivering an RBC transfusion that develops NEC.
贫血是一个常见且严重的健康问题，几乎在所有早产儿中都会被诊断出来，并且在全球范围内与发病率和死亡率的增加相关。红细胞（RBC）输血是一种挽救生命的主要治疗方法；然而，它有严重的不良反应。其中一个后果是坏死性小肠结肠炎（NEC），这是一种早产儿的炎症性肠坏死疾病。放血诱导贫血和红细胞输血相关NEC的小鼠模型能够详细研究这些疾病背后的分子机制，并评估潜在的新治疗策略。本方案描述了获得放血诱导贫血的幼鼠并进行红细胞输血以引发NEC的详细程序。
