Premoli Marika, Carone Marinella, Mastinu Andrea, Maccarinelli Giuseppina, Aria Francesca, Mac Sweeney Eileen, Memo Maurizio, Bonini Sara Anna
Department of Molecular and Translational Medicine, University of Brescia, Brescia, Italy.
Cannabis Cannabinoid Res. 2024 Dec;9(6):1514-1523. doi: 10.1089/can.2024.0062. Epub 2024 May 27.
Cannabis sativa is the most used recreational drug worldwide. In recent years, there has been a growing interest in the potential therapeutic benefits of medicinal cannabis to treat a variety of psychiatric and neurological conditions. In particular, cannabidiol (CBD), a nonpsychoactive cannabis constituent, has been investigated for its potential prosocial effects on behavior, although the molecular mechanisms underlying this effect are still largely unknown. The aim of this study was to investigate the effect of a C. sativa oil CBD rich (CS oil) on social interaction and ultrasonic communication in mice. Twenty-seven adult male mice (B6; 129P F2) were treated daily with vehicle or CS oil for 2 weeks. At Day 14, mice were tested for behavior (social interaction test and ultrasonic communication). Forty minutes before the behavioral tests, mice were exposed to intranasal treatment with vehicle or the oxytocin receptor antagonist, L-371,257. After behavioral tests, VH- and CS oil-treated mice were sacrificed, RNA was extracted from the hypothalamus and used for quantitative Real Time-PCR experiments. We found that a 2-week treatment with the CS oil on mice exerted a prosocial effect associated with an increase in ultrasonic vocalizations. These effects were inhibited by pretreating mice with an oxytocin receptor antagonist. In addition, at the molecular level, we found that CS oil treatment caused a significant increase in oxytocin and a decrease in oxytocin receptor expression levels in the brain hypothalamus. Our results suggest that CS oil promotes social behavior by acting on oxytocin pathway.
大麻是全球使用最广泛的消遣性毒品。近年来，人们对药用大麻治疗各种精神和神经疾病的潜在治疗益处的兴趣与日俱增。特别是，大麻二酚（CBD），一种无精神活性的大麻成分，已因其对行为的潜在亲社会效应而受到研究，尽管这种效应背后的分子机制仍 largely unknown。本研究的目的是调查富含大麻二酚的大麻籽油（CS油）对小鼠社交互动和超声通讯的影响。27只成年雄性小鼠（B6；129P F2）每天接受载体或CS油处理，持续2周。在第14天，对小鼠进行行为测试（社交互动测试和超声通讯）。在行为测试前40分钟，小鼠接受鼻腔内载体或催产素受体拮抗剂L-371,257的处理。行为测试后，处死接受载体和CS油处理的小鼠，从下丘脑提取RNA并用于定量实时PCR实验。我们发现，对小鼠进行2周的CS油处理产生了与超声发声增加相关的亲社会效应。这些效应被用催产素受体拮抗剂预处理小鼠所抑制。此外，在分子水平上，我们发现CS油处理导致大脑下丘脑催产素显著增加，催产素受体表达水平降低。我们的结果表明，CS油通过作用于催产素途径促进社交行为。
