Nanjing Center for Disease Control and Prevention, Nanjing, Jiangsu Province, China.
Eur J Clin Microbiol Infect Dis. 2024 Jul;43(7):1445-1452. doi: 10.1007/s10096-024-04858-z. Epub 2024 May 27.
The objective of this study was to examine the molecular epidemiology and clinical characteristics of HMPV infection among children with ARIs in Nanjing.
The respiratory samples were collected from 2078 children (≤ 14 years) with acute respiratory infections and were tested for HMPV using real-time RT-PCR. Amplification and sequencing of the HMPV G gene were followed by phylogenetic analysis using MEGA 7.0.
The detection rate of HMPV among children was 4.7% (97/2078), with a concentration in those under 5 years of age. Notably, the peak season for HMPV prevalence was observed in winter. Among the 97 HMPV-positive samples, 51.5% (50/97) were available for characterization of the HMPV G protein gene. Phylogenetic analysis indicated that the sequenced HMPV strains were classified into three sublineages: A2c (84.0%), B1 (2.0%), and B2 (14.0%).
There was an incidence of HMPV among hospitalized children during 2021-2022 in Nanjing with A2c being the dominant strain. This study demonstrated the molecular epidemiological characteristics of HMPV among children with respiratory infections in Nanjing, China.
本研究旨在探讨南京地区急性呼吸道感染（ARI）患儿中人偏肺病毒（HMPV）感染的分子流行病学和临床特征。
收集 2078 例急性呼吸道感染患儿的呼吸道样本，采用实时 RT-PCR 检测 HMPV。对 HMPV G 基因进行扩增和测序，然后使用 MEGA 7.0 进行系统进化分析。
儿童中 HMPV 的检出率为 4.7%（97/2078），以 5 岁以下儿童为主。值得注意的是，HMPV 的流行高峰出现在冬季。在 97 例 HMPV 阳性样本中，有 51.5%（50/97）可用于 HMPV G 蛋白基因特征分析。系统进化分析表明，测序的 HMPV 株分为三个亚系：A2c（84.0%）、B1（2.0%）和 B2（14.0%）。
2021-2022 年南京住院儿童中存在 HMPV 感染，A2c 是主要流行株。本研究表明了中国南京地区呼吸道感染患儿中 HMPV 的分子流行病学特征。
