Division of Immunology, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing, China.
J Clin Microbiol. 2012 Aug;50(8):2714-9. doi: 10.1128/JCM.00809-12. Epub 2012 Jun 12.
Human metapneumovirus (hMPV) is the main pathogen causing respiratory tract infection in susceptible populations, particularly in children and the elderly. Specimens were collected from hospitalized children with acute lower respiratory tract infections (ALRTI), and the hMPV was detected by using real-time reverse transcription-PCR (RT-PCR). The full-length G gene of hMPV was amplified by RT-PCR. A total of 1,410 nasopharyngeal aspirates (NPAs) were collected from April 2008 to March 2011, and 114 (10.2%) were positive for hMPV. Most hMPV-positive children were <5 years of age. The hMPV infection rate peaked in the spring-summer season of 2008 to 2009 and 2009 to 2010, while hMPV circulated predominantly during the winter-spring season of 2010 to 2011. The full-length G gene of 23 hMPV strains was amplified, and group A and B viruses accounted for 95.7% (22/23) and 4.3% (1/23), respectively. Genotype A2b of hMPV appeared to be predominant during the study period. Three genotypes (A2b, A1, and B1) were prevalent in the epidemic season of 2008 to 2009, and only genotype A2b was identified in the other two seasons (2009 to 2010 and 2010 to 2011). The G gene of hMPV was predicted to encode proteins with four different lengths, in which one with 210 amino acids was first identified in China. These findings suggest that hMPV was an important pathogen of ALRTI in pediatric patients, especially those <5 years of age. Genotype A2b of hMPV likely predominates in Southwest China, where other genotypes also circulate.
人偏肺病毒（hMPV）是引起易感人群呼吸道感染的主要病原体，尤其是儿童和老年人。采集因急性下呼吸道感染（ALRTI）住院的儿童的标本，并用实时逆转录-PCR（RT-PCR）检测 hMPV。通过 RT-PCR 扩增 hMPV 的全长 G 基因。共采集 2008 年 4 月至 2011 年 3 月的 1410 份鼻咽抽吸物（NPA），其中 114 份（10.2%）hMPV 阳性。大多数 hMPV 阳性患儿年龄<5 岁。hMPV 感染率在 2008 至 2009 年和 2009 至 2010 年的春夏季节达到高峰，而 hMPV 主要在 2010 至 2011 年的冬春季流行。扩增了 23 株 hMPV 全长 G 基因，A 组和 B 组病毒分别占 95.7%（22/23）和 4.3%（1/23）。研究期间，hMPV 出现了 A2b 型优势株。三种基因型（A2b、A1 和 B1）在 2008 至 2009 年流行季节流行，而在其他两个季节（2009 至 2010 年和 2010 至 2011 年）仅鉴定出基因型 A2b。hMPV 的 G 基因预测编码四种不同长度的蛋白，其中一种含 210 个氨基酸的蛋白在中国首次被鉴定。这些发现表明 hMPV 是儿科 ALRTI 的重要病原体，尤其是<5 岁的患儿。hMPV 的 A2b 基因型可能在中国西南地区占优势，其他基因型也在该地区流行。
