综合分析表明ANKRD53在胃腺癌中的潜在价值。
Integrative analysis indicates the potential values of ANKRD53 in stomach adenocarcinoma.
作者信息
Jin Chunjing, Lu Xu, Yang Minfeng, Hou Shiqiang
机构信息
Laboratory Medicine Center, The Affiliated Chuzhou Hospital of Anhui Medical University, The First People's Hospital of Chuzhou, Chuzhou, China.
Department of General Surgery, The Affiliated Chuzhou Hospital of Anhui Medical University, The First People's Hospital of Chuzhou, Chuzhou, China.
出版信息
Discov Oncol. 2024 May 27;15(1):188. doi: 10.1007/s12672-024-01054-5.
BACKGROUND
Ankyrin repeat domain 53 (ANKRD53) plays an important role in maintaining chromosome integrity and stability, and chromosome instability is associated with cancer. Through integrative analysis, this study investigates the potential value of ANKRD53 in stomach adenocarcinoma (STAD).
METHODS
RNA-seq and scRNA-seq data were used for integrative analysis based on online databases. Expression of ANKRD53 was confirmed by RT-PCR after bioinformatic analysis. Kaplan-Meier and Cox regression analyses were performed to evaluate the prognostic value of ANKRD53 in STAD. Gene set enrichment analysis (GSEA) was performed to evaluate ANKRD53-related signaling pathways. In addition, the interaction of ANKRD53 with immunity was also investigated.
RESULTS
RT-PCR in STAD cell lines confirmed that ANKRD53 was downregulated in STAD samples compared to normal samples in the online databases. As an independent predictive biomarker, ANKRD53 was combined with other clinicopathological parameters to create a prognostic nomogram. Using GSEA, ANKRD53 was found to be involved in five pathways, including the TGF-β signaling pathway. Further investigation revealed that ANKRD53 was associated with immune checkpoint molecules, immunological pathways, and immunotherapy, in addition to MSI, TMB and neoantigens. In addition, scRNA-seq data revealed that ANKRD53 is mainly expressed in CD8 T and dendritic cells.
CONCLUSIONS
ANKRD53 is an important biomarker for STAD that deserves further attention.
背景
锚蛋白重复结构域53（ANKRD53）在维持染色体完整性和稳定性方面发挥着重要作用，而染色体不稳定与癌症相关。通过综合分析，本研究探讨ANKRD53在胃腺癌（STAD）中的潜在价值。
方法
基于在线数据库，使用RNA测序和单细胞RNA测序数据进行综合分析。经过生物信息学分析后，通过逆转录聚合酶链反应（RT-PCR）确认ANKRD53的表达。进行Kaplan-Meier分析和Cox回归分析，以评估ANKRD53在STAD中的预后价值。进行基因集富集分析（GSEA），以评估与ANKRD53相关的信号通路。此外，还研究了ANKRD53与免疫的相互作用。
结果
STAD细胞系中的RT-PCR证实，与在线数据库中的正常样本相比，ANKRD53在STAD样本中表达下调。作为一个独立的预测生物标志物，ANKRD53与其他临床病理参数相结合，创建了一个预后列线图。使用GSEA发现ANKRD53参与了五个信号通路，包括转化生长因子-β（TGF-β）信号通路。进一步研究发现，ANKRD53除了与微卫星不稳定性（MSI）、肿瘤突变负荷（TMB）和新抗原有关外，还与免疫检查点分子、免疫途径和免疫治疗相关。此外，单细胞RNA测序数据显示ANKRD53主要在CD8 T细胞和树突状细胞中表达。
结论
ANKRD53是STAD的一个重要生物标志物，值得进一步关注。
Zotero 插件