Department of Pharmaceutical Botany and Authentication of TCM, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
Department of Pharmaceutical Botany and Authentication of TCM, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China..
Fitoterapia. 2024 Sep;177:106043. doi: 10.1016/j.fitote.2024.106043. Epub 2024 May 25.
Three undescribed phenols, mandshusica C-E (1-3) and a new lignan, mandshusica F (5), along with six known compounds (4, 6-10) were isolated from the roots and rhizomes of Clematis terniflora var. manshurica (Rupr.) Ohwi. Their structures were elucidated by extensive spectroscopic analysis as well as NMR and ECD calculations. Moreover, the possible biosynthetic pathways of compounds 1-3 were also discussed. All compounds were evaluated for their anti-inflammatory activities in LPS-induced RAW 264.7 cells. Compounds 1, 3, 4 significantly reduced the levels of NO and TNF-α, while compounds 2 and 8 significantly inhibited NO production in LPS-induced RAW264.7 cells.
从毛茛科铁线莲属植物展枝铁线莲（Clematis terniflora var. manshurica）的根和根茎中分离得到了三种未被描述的酚类化合物，曼舒斯卡 C-E（1-3）和一种新的木脂素，曼舒斯卡 F（5），以及六种已知化合物（4、6-10）。通过广泛的光谱分析以及 NMR 和 ECD 计算，阐明了它们的结构。此外，还讨论了化合物 1-3 的可能生物合成途径。所有化合物均在 LPS 诱导的 RAW 264.7 细胞中进行了抗炎活性评价。化合物 1、3、4 显著降低了 NO 和 TNF-α 的水平，而化合物 2 和 8 则显著抑制了 LPS 诱导的 RAW264.7 细胞中 NO 的产生。
