State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China.
State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China.
Bioresour Technol. 2024 Jul;403:130903. doi: 10.1016/j.biortech.2024.130903. Epub 2024 May 25.
Sulfate-dependent ammonium oxidation (Sulfammox) is a critical process linking nitrogen and sulfur cycles. However, the metabolic pathway of microbes driven Sulfammox is still in suspense. The study demonstrated that ammonium was not consumed with sulfate as the sole electron acceptor during long-term enrichment, probably due to inhibition from sulfide accumulation, while ammonium was removed at ∼ 10 mg N/L/d with sulfate and nitrate as electron acceptors. Ammonium and sulfate were converted into nitrogen gas, sulfide, and elemental sulfur. Sulfammox was mainly performed by Candidatus Brocadia sapporoensis and Candidatus Brocadia fulgida, both of which encoded ammonium oxidation pathway and dissimilatory sulfate reduction pathway. Not sulfide-driven autotrophic denitrifiers but Candidatus Kuenenia stuttgartiensis converted nitrate to nitrite with sulfide. The results of this study reveal the specialized metabolism of Sulfammox bacteria (Candidatus Brocadia sapporoensis and Candidatus Brocadia fulgida) and provide insight into microbial relationships during the nitrogen and sulfur cycles.
硫酸盐依赖型氨氧化（Sulfammox）是连接氮硫循环的关键过程。然而，驱动 Sulfammox 的微生物的代谢途径仍悬而未决。研究表明，在长期富集过程中，当硫酸盐作为唯一的电子受体时，氨并没有被消耗，这可能是由于硫化物积累的抑制作用，而当硫酸盐和硝酸盐作为电子受体时，氨以约 10mgN/L/d 的速率被去除。氨和硫酸盐转化为氮气、硫化物和元素硫。Sulfammox 主要由 Candidatus Brocadia sapporoensis 和 Candidatus Brocadia fulgida 进行，这两种菌都编码氨氧化途径和异化硫酸盐还原途径。将硝酸盐转化为亚硝酸盐的不是受硫化物驱动的自养反硝化菌，而是 Candidatus Kuenenia stuttgartiensis，其利用硫化物进行反硝化作用。本研究揭示了 Sulfammox 细菌（Candidatus Brocadia sapporoensis 和 Candidatus Brocadia fulgida）的特殊代谢，并深入了解了氮硫循环过程中的微生物关系。
