Novak R J, Moss D, Byrd H
Division of Vector-Borne Viral Diseases, Centers for Disease Control, San Juan, PR 00936.
J Am Mosq Control Assoc. 1985 Sep;1(3):353-6.
A procedure for high resolution electrophoresis of isozymes of the mosquito Aedes aegypti using nonlinear (2.5%-20%) polyacrylamide gradient gel slabs is described. Crude mosquito homogenates were electrophoresed on gradient and on homogeneous (7%) polyacrylamide gels and stained for esterase (EST), isocitrate dehydrogenase (IDH) and malate dehydrogenase (MDH). The resulting zymograms were compared to demonstrate the high resolving power of the polyacrylamide gradient gel system.
本文描述了一种使用非线性（2.5%-20%）聚丙烯酰胺梯度凝胶板对埃及伊蚊同工酶进行高分辨率电泳的方法。将粗制的蚊虫匀浆在梯度和均匀（7%）聚丙烯酰胺凝胶上进行电泳，并对酯酶（EST）、异柠檬酸脱氢酶（IDH）和苹果酸脱氢酶（MDH）进行染色。比较所得的酶谱，以证明聚丙烯酰胺梯度凝胶系统的高分辨率能力。
