Karssen G, van Hoenselaar T, Verkerk-Bakker B, Janssen R
Plant Protection Service, Nematology Section, Wageningen, The Netherlands.
Electrophoresis. 1995 Jan;16(1):105-9. doi: 10.1002/elps.1150160119.
To carry out rapid and reliable species identification of Globodera and Meloidogyne specimens, two electrophoretic methods were modified and adapted for an automated electrophoresis system (PhastSystem, Pharmacia). Proteins of individual Globodera cysts were identified using isoelectric focusing and a sensitive silver stain. Proteins of young single Meloidogyne females were separated using polyacrylamide gradient gels and stained for the isozymes esterase and malate dehydrogenase.
为了对球孢囊线虫属和根结线虫属标本进行快速可靠的物种鉴定，对两种电泳方法进行了改进，以适用于自动化电泳系统（Pharmacia公司的PhastSystem）。利用等电聚焦和灵敏的银染法鉴定单个球孢囊线虫属孢囊的蛋白质。利用聚丙烯酰胺梯度凝胶分离年轻的单个根结线虫属雌虫的蛋白质，并对酯酶和苹果酸脱氢酶同工酶进行染色。
