Suzhou Key Laboratory of Nanotechnology and Biomedicine, Institute of Functional Nano and Soft Materials (FUNSOM), and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215123, China.
State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Soochow University, Suzhou 215123, China.
Anal Chem. 2024 Jun 11;96(23):9684-9692. doi: 10.1021/acs.analchem.4c01626. Epub 2024 May 28.
Herein, we report a DNA origami plasmonic nanoantenna for the programmable surface-enhanced Raman scattering (SERS) detection of cytokine release syndrome (CRS)-associated cytokines (e.g., tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ)) in cancer immunotherapy. Typically, the nanoantenna was made of self-assembled DNA origami nanotubes (diameter: ∼19 nm; length: ∼90 nm) attached to a silver nanoparticle-modified silicon wafer (AgNP/Si). Each DNA origami nanotube contains one miniature gold nanorod (AuNR) inside (e.g., length: ∼35 nm; width: ∼7 nm). Intriguingly, TNF-α and IFN-γ logically regulate the opening of the nanotubes and the dissociation of the AuNRs from the origami structure upon binding to their corresponding aptamers. On this basis, we constructed a complete set of Boolean logic gates that read cytokine molecules as inputs and return changes in Raman signals as outputs. Significantly, we demonstrated that the presented system enables the quantification of TNF-α and IFN-γ in the serum of tumor-bearing mice receiving different types of immunotherapies (e.g., PD1/PD-L1 complex inhibitors and STING agonists). The sensing results are consistent with those of the ELISA. This strategy fills a gap in the use of DNA origami for the detection of multiple cytokines in real systems.
在此，我们报告了一种 DNA 折纸等离子体纳米天线，用于癌症免疫治疗中细胞因子释放综合征 (CRS) 相关细胞因子（例如肿瘤坏死因子-α (TNF-α) 和干扰素-γ (IFN-γ)）的可编程表面增强拉曼散射 (SERS) 检测。通常，纳米天线由自组装的 DNA 折纸纳米管（直径：19nm；长度：90nm）组成，附着在银纳米颗粒修饰的硅片（AgNP/Si）上。每个 DNA 折纸纳米管内部都包含一个微型金纳米棒（AuNR）（例如，长度：35nm；宽度：7nm）。有趣的是，TNF-α 和 IFN-γ 通过与其相应的适体结合，逻辑上调节纳米管的打开和 AuNR 从折纸结构的解离。在此基础上，我们构建了一套完整的布尔逻辑门，将细胞因子分子作为输入，并将拉曼信号的变化作为输出返回。重要的是，我们证明了该系统能够定量检测接受不同类型免疫疗法（例如 PD1/PD-L1 复合物抑制剂和 STING 激动剂）的荷瘤小鼠血清中的 TNF-α 和 IFN-γ。传感结果与 ELISA 的结果一致。该策略填补了 DNA 折纸在真实系统中检测多种细胞因子的空白。
