School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, 730050, PR China.
Key Laboratory of Drug Screening and Deep Processing for Traditional Chinese and Tibetan Medicine of Gansu Province, Lanzhou, 730050, PR China.
Chem Biodivers. 2024 Aug;21(8):e202400596. doi: 10.1002/cbdv.202400596. Epub 2024 Jul 6.
Three polysaccharides, PTC, PTH, and PTB, were extracted from Pinellia ternata using three different extraction conditions: room temperature water, hot water, and 2 % NaCO solution. PTC and PTH were composed of rhamnose, glucose, galactose, mannose, glucuronic acid, galacturonic acid, and arabinose, which combine to form complex structures. PTB was composed solely of glucose and rhamnose. Further analysis indicated that PTC and PTB exhibited triple-helix structures. PTC showed the highest scavenging capacity against DPPH, superoxide anion, and hydroxyl radicals, with half maximal inhibitory concentrations (IC) of 1004.1, 1584.1, and 1584.1 μg/mL, respectively. Additionally, PTC, PTH, and PTB were subjected to sulfation, phosphorylation, and selenization, resulting in the production of nine derivates. The distinctive absorptive bands of these derivates were determined through infrared spectroscopy. Selenized and sulfated derivates have shown significant antitumor and immunoenhancing properties. Our findings revealed that at 400 μg/mL, the inhibition rate of selenated PTB on HeLa cells was 54.2 % and that on HepG2 cells was 43.1 %. Additionally, selenized PTC displayed significant immunoenhancing activity, with a proliferation rate of 63.7 % at 400 μg/mL in RAW264.7 cells. These results provide valuable evidence supporting the consideration of polysaccharides from Pinellia ternata as a potential candidate for the development of antineoplastic drugs.
从半夏中提取了三种多糖，即 PTC、PTH 和 PTB，采用了三种不同的提取条件：室温水、热水和 2%NaCO 溶液。PTC 和 PTH 由鼠李糖、葡萄糖、半乳糖、甘露糖、葡萄糖醛酸、半乳糖醛酸和阿拉伯糖组成，它们结合形成复杂的结构。PTB 仅由葡萄糖和鼠李糖组成。进一步分析表明，PTC 和 PTB 具有三螺旋结构。PTC 对 DPPH、超氧阴离子和羟基自由基的清除能力最强，半最大抑制浓度（IC）分别为 1004.1、1584.1 和 1584.1μg/mL。此外，对 PTC、PTH 和 PTB 进行了硫酸化、磷酸化和硒化，得到了 9 种衍生物。通过红外光谱确定了这些衍生物的特征吸收带。硒化和硫酸化衍生物表现出显著的抗肿瘤和免疫增强特性。我们的研究结果表明，在 400μg/mL 时，硒化 PTB 对 HeLa 细胞的抑制率为 54.2%，对 HepG2 细胞的抑制率为 43.1%。此外，硒化 PTC 表现出显著的免疫增强活性，在 RAW264.7 细胞中，400μg/mL 时的增殖率为 63.7%。这些结果为将半夏多糖作为抗肿瘤药物开发的潜在候选物提供了有价值的证据。
