Figueroa Fabián A, Abdala-Diáz Roberto, Cabrera-Barjas Gustavo, Lopez Fernanda, Moncada-Basualto Mauricio, Parra-Magna Sebastián, Castro-Varela Pablo, Montecinos Lya, Quintana Froilán, Peréz Claudia, Becerra José
Laboratorio de Química de Productos Naturales, Departamento de Botánica, Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción, Casilla 160-C, Concepción 4030000, Chile; Facultad de Ciencias, Universidad Católica de la Santísima Concepción, Concepción, Chile.
Instituto Andaluz de Biotecnología y Desarrollo Azul (IBYDA), Departamento de Ecología, Facultad de Ciencias, Universidad de Málaga, Campus de Teatinos s/n, CP 29071, Málaga, Spain.
Int J Biol Macromol. 2025 Jul;318(Pt 1):144978. doi: 10.1016/j.ijbiomac.2025.144978. Epub 2025 Jun 4.
In this study the chemical composition of fucoidans from Macrocystis pyrifera seaweed collected along a latitudinal gradient on the Chilean coast was investigated. Three water-soluble fucoidans were extracted and characterized using different physico-chemical techniques. Their antioxidant, anticoagulant, and cytotoxic activities were also assessed. The results showed that three extracted fucoidans contained a high degree of sulfation and had a high molecular weight. The sugar analysis revealed the presence of fucose, mannose, galactose, glucuronic acid, and glucose monosaccharides at different molar ratios, depending on the collection site, with Fuc:Man ratio ranging from 7.7. to 17.3. 2D NMR analysis suggested that M. pyrifera contained a fucoidan with a highly regular α-1 → 3 glycosidic bond, which was completely sulfated at the O-4 position and partially sulfated at O-2. The anticoagulant activity test showed that the clotting time for the three fucoidans was longer at concentrations of 100 and 500 μg mL. Fucoidans exhibited moderate to low antioxidant activity. Finally, a high selectivity index (>3) for all fucoidans on P-388 leukemia cancer cells was obtained. These results suggest that fucoidans extracted from M. pyrifera have a high potential as natural anticoagulants, and antitumoral agents for industrial applications.
在本研究中，对沿智利海岸纬度梯度采集的巨藻（Macrocystis pyrifera）中海藻糖胶的化学成分进行了研究。提取了三种水溶性海藻糖胶，并使用不同的物理化学技术对其进行了表征。还评估了它们的抗氧化、抗凝血和细胞毒性活性。结果表明，三种提取的海藻糖胶具有高度的硫酸化程度和高分子量。糖分析显示，根据采集地点的不同，岩藻糖、甘露糖、半乳糖、葡萄糖醛酸和葡萄糖单糖以不同的摩尔比存在，岩藻糖与甘露糖的比例在7.7至17.3之间。二维核磁共振分析表明，巨藻含有一种具有高度规则的α-1→3糖苷键的海藻糖胶，其在O-4位完全硫酸化，在O-2位部分硫酸化。抗凝血活性测试表明，在100和500μg/mL的浓度下，三种海藻糖胶的凝血时间更长。海藻糖胶表现出中度至低度的抗氧化活性。最后，所有海藻糖胶对P-388白血病癌细胞的选择性指数都很高（>3）。这些结果表明，从巨藻中提取的海藻糖胶具有作为天然抗凝血剂和工业应用抗肿瘤剂的巨大潜力。
