Xue Tao-Tao, Zheng Dong-Xuan, Hou Qiang, Wen Li-Mei, Wang Bao-Juan, Geng Ruo-Yu, Wang Qian-Qian, Dai Wu, Tian Li-Ying, He Sheng-Qi, Yang Jian-Hua, Hu Jun-Ping
College of Pharmacy, Xinjiang Medical University, Urumqi, China.
Department of Pharmacy, The First Affiliated Hospital, Xinjiang Medical University, Urumqi, China.
Phytochem Anal. 2025 Jul;36(5):1333-1350. doi: 10.1002/pca.3512. Epub 2025 Jan 23.
Cistanche deserticola Ma (CD), an edible and medicinal plant native to Xinjiang, Inner Mongolia, and Gansu in China, is rich in bioactive polysaccharides known for their health-promoting properties. The polysaccharides of C. deserticola (CDPs) have been shown to possess a range of beneficial activities, including immunomodulatory, anti-aging, antioxidant, and anti-osteoporosis effects.
This study seeks to identify the optimal conditions for extracting CDPs using hot water. Additionally, it aims to evaluate their chemical properties, antioxidant activity, hypoglycemic effects, and cytotoxicity. The findings will provide a theoretical foundation for the potential use of CDPs in functional foods and pharmaceuticals.
The study employed response surface methodology to optimize the hot water extraction conditions for CDPs. The extracted CDPs were characterized using a range of chemical, spectroscopic, and instrumental analyses. Furthermore, their antioxidant activity, hypoglycemic effects, and cytotoxicity were evaluated through relevant assays to assess their potential health benefits.
Under optimal conditions, the yield of CDPs was 45.85% ± 1.91%. CDPs were identified as acidic heteropolysaccharides with a wide molecular weight distribution, ranging from 0.3 to 128.2 kDa. They were composed primarily of glucose (51.21%), arabinose (32.86%), galactose (17.88%), and smaller amounts of galacturonic acid (4.66%), rhamnose (1.85%), mannose (1.32%), glucosamine hydrochloride (1.08%), and xylose (0.56%). Antioxidant assays demonstrated that CDPs exhibited significant free radical scavenging activity, metal ion chelation, and reducing power. Additionally, CDPs inhibited α-glucosidase and α-amylase in vitro through a mixed-type mechanism, as well as static fluorescence quenching. Cytotoxicity assays showed that CDPs were nontoxic to L02 and AML12 cells.
This study offers a theoretical foundation for the potential use of CDPs in functional foods and pharmaceuticals and provides valuable insights for the development of new antioxidant and hypoglycemic agents from natural sources.
肉苁蓉是一种原产于中国新疆、内蒙古和甘肃的药食两用植物，富含具有保健特性的生物活性多糖。肉苁蓉多糖（CDPs）已被证明具有一系列有益活性，包括免疫调节、抗衰老、抗氧化和抗骨质疏松作用。
本研究旨在确定热水提取CDPs的最佳条件。此外，旨在评估其化学性质、抗氧化活性、降血糖作用和细胞毒性。研究结果将为CDPs在功能性食品和药物中的潜在应用提供理论基础。
本研究采用响应面法优化CDPs的热水提取条件。使用一系列化学、光谱和仪器分析对提取的CDPs进行表征。此外，通过相关试验评估其抗氧化活性、降血糖作用和细胞毒性，以评估其潜在的健康益处。
在最佳条件下，CDPs的得率为45.85%±1.91%。CDPs被鉴定为酸性杂多糖，分子量分布较宽，范围为0.3至128.2 kDa。它们主要由葡萄糖（51.21%）、阿拉伯糖（32.86%）、半乳糖（17.88%）以及少量的半乳糖醛酸（4.66%）、鼠李糖（1.85%）、甘露糖（1.32%）、盐酸氨基葡萄糖（1.08%）和木糖（0.56%）组成。抗氧化试验表明，CDPs表现出显著的自由基清除活性、金属离子螯合能力和还原能力。此外，CDPs在体外通过混合型机制抑制α-葡萄糖苷酶和α-淀粉酶，以及静态荧光猝灭。细胞毒性试验表明，CDPs对L02和AML12细胞无毒。
本研究为CDPs在功能性食品和药物中的潜在应用提供了理论基础，并为从天然来源开发新型抗氧化剂和降血糖药物提供了有价值的见解。
