Hamada Y, Yamamura M, Hioki K, Yamamoto M, Nagura H, Watanabe K
Cancer. 1985 Jan 1;55(1):136-41. doi: 10.1002/1097-0142(19850101)55:1<136::aid-cncr2820550121>3.0.co;2-p.
Using peroxidase-antiperoxidase (PAP) method, carcinoembryonic antigen (CEA) was demonstrated in conventionally processed colorectal cancer tissues. A new immunohistochemical grading for colorectal cancers based on the mode of the localization was made in an attempt to clarify the factors responsible for elevation of plasma CEA levels in colorectal cancer patients. Most of the patients with well differentiated adenocarcinoma, in which CEA was densely distributed along the apical surface but only rarely present along the basolateral surfaces of the carcinoma cells, had very low levels of plasma CEA, whereas all patients showing CEA distribution in the stroma as well as over the entire surfaces of the cancer cells and their cytoplasm showed high plasma CEA levels. In addition, there was a good correlation between the grading and presence of the blood vessel and lymphatic invasions. Thus, the appearance of CEA in the surrounding stroma, due to abnormal distribution of CEA on the basolateral plasma membrane of cancer cells, may play a significant role in the elevation of plasma CEA levels in colorectal cancer patients.
采用过氧化物酶-抗过氧化物酶（PAP）法，在常规处理的结直肠癌组织中检测到癌胚抗原（CEA）。基于CEA定位模式对结直肠癌进行了一种新的免疫组织化学分级，旨在阐明导致结直肠癌患者血浆CEA水平升高的因素。大多数高分化腺癌患者，其CEA沿癌细胞顶端表面密集分布，但沿基底外侧表面很少出现，血浆CEA水平很低，而所有在基质以及癌细胞及其细胞质整个表面均显示CEA分布的患者，血浆CEA水平都很高。此外，分级与血管及淋巴管侵犯的存在之间存在良好的相关性。因此，由于癌细胞基底外侧质膜上CEA分布异常导致其在周围基质中出现，可能在结直肠癌患者血浆CEA水平升高中起重要作用。
