School of Life Science and Technology, China Pharmaceutical University, 639 Longmian Road, Nanjing, Jiangsu Province, 211198, China.
Lei Yun Shang Pharmaceutical Group Co., Ltd, Suzhou, 215009, China.
Arch Virol. 2024 May 28;169(6):130. doi: 10.1007/s00705-024-06053-z.
Qingke Pingchuan granules (QPGs), which contain Houttuynia cordata Thunb, Fritillaria cirrhosa, fired licorice, and fired bitter almonds, among other components, can clear heat and ventilate the lungs, relieving cough and asthma. Clinically, QPGs are mainly used to treat cough, asthma, fever and other discomforts caused by acute or chronic bronchitis. In this study, the antiviral activity of QPGs against respiratory syncytial virus (RSV), influenza A virus A/FM/1/47 (H1N1), oseltamivir-resistant H1N1, A/Beijing/32/92 (H3N2), Sendai virus, and human adenovirus type 3 in Hep-2 or MDCK cells was evaluated using the CCK-8 method, and the cytotoxicity of QPGs to these two cell lines was tested. The effect of QPGs on mice infected with influenza A virus A/FM/1/47 (H1N1) was evaluated by measuring body weight, survival time, and survival rate, as well as virus titers and lesions in the lungs and levels of inflammatory factors in serum. In addition, the expression of TLR-7-My88-NF-κB signaling pathway-related proteins in lung tissues was analyzed by Western blotting and qRT-PCR. The results showed that QPGs had a potent inhibitory effect on the six viruses tested in vitro. Interestingly, QPGs also displayed particularly pronounced antiviral activity against H1N1-OC, similar to that of oseltamivir, a well-known antiviral drug. QPGs effectively protected mice from infection by H1N1, as indicated by significantly increased body weights, survival times, and survival rates and reduced lung virus titers of inflammatory factors and lung tissue injury. The levels of TLR-7-MyD88-NF-κB-pathway-related proteins in the lung tissue of infected mice were found to be decreased after QPG treatment, thereby alleviating lung injury caused by excessive release of inflammatory factors. Taken together, these findings indicate that QPGs have satisfactory activity against influenza virus infection.
清咳平喘颗粒（QPGs），包含鱼腥草、款冬花、炙甘草和苦杏仁等成分，可清热宣肺，止咳平喘。临床上，QPGs 主要用于治疗急性或慢性支气管炎引起的咳嗽、哮喘、发热等不适。在这项研究中，采用 CCK-8 法评价 QPGs 对呼吸道合胞病毒（RSV）、甲型流感病毒 A/FM/1/47（H1N1）、奥司他韦耐药 H1N1、A/Beijing/32/92（H3N2）、仙台病毒和人腺病毒 3 型在 Hep-2 或 MDCK 细胞中的抗病毒活性，并测试了 QPGs 对这两种细胞系的细胞毒性。通过测量体重、存活时间和存活率以及病毒滴度和肺损伤以及血清中炎症因子水平，评价 QPGs 对感染甲型流感病毒 A/FM/1/47（H1N1）的小鼠的作用。此外，通过 Western blot 和 qRT-PCR 分析肺组织中 TLR-7-My88-NF-κB 信号通路相关蛋白的表达。结果表明，QPGs 在体外对六种测试病毒具有强大的抑制作用。有趣的是，QPGs 对 H1N1-OC 也表现出特别明显的抗病毒活性，与奥司他韦等知名抗病毒药物相当。QPGs 有效地保护了小鼠免受 H1N1 的感染，表现为体重、存活时间和存活率显著增加，肺病毒滴度和炎症因子以及肺组织损伤降低。感染小鼠肺组织中 TLR-7-MyD88-NF-κB 通路相关蛋白的水平在 QPG 治疗后降低，从而减轻了炎症因子过度释放引起的肺损伤。综上所述，这些发现表明 QPGs 对流感病毒感染具有令人满意的活性。
