Burgoyne R D, Norman K M
FEBS Lett. 1985 Jan 1;179(1):25-8. doi: 10.1016/0014-5793(85)80183-6.
The presence of tropomyosins in adrenal chromaffin cells was demonstrated by immunoperoxidase staining of cultured chromaffin cells by an anti-tropomyosin antibody. In immunoblotting the antibody labelled polypeptides of Mr 39 000, 36 500 and 31 000 in 100 000 X g supernatants and heat-stable fractions of adrenal medulla. The adrenal medullary tropomyosins did not comigrate with smooth muscle tropomyosins. The 39-kDa and 31-kDa polypeptides comigrated with polypeptides labelled by anti-tropomyosin in brain supernatants. Chromaffin granule membranes possessed a 39-kDa polypeptide that was labelled by anti-tropomyosin.
通过用抗原肌球蛋白抗体对培养的嗜铬细胞进行免疫过氧化物酶染色，证实了肾上腺嗜铬细胞中存在原肌球蛋白。在免疫印迹中，该抗体标记了肾上腺髓质100000×g上清液和热稳定组分中分子量为39000、36500和31000的多肽。肾上腺髓质原肌球蛋白与平滑肌原肌球蛋白迁移率不同。39-kDa和31-kDa多肽与脑上清液中抗原肌球蛋白标记的多肽迁移率相同。嗜铬颗粒膜含有一种被抗原肌球蛋白标记的39-kDa多肽。
