Zeng Tiexin, Ni Yang, Li Jingling, Chen Haimei, Lu Qianqi, Jiang Mei, Xu Lijia, Liu Chang, Xiao Peigen
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
Front Plant Sci. 2024 May 14;15:1326387. doi: 10.3389/fpls.2024.1326387. eCollection 2024.
is an economically significant medicinal plant. Yet, the structure and sequence of its mitochondrial genome has not been published, which plays a crucial role in evolutionary analysis and regulating respiratory-related macromolecule synthesis. In this study, the mitogenome was sequenced employing a combination of Illumina short reads and Nanopore long reads, with subsequent assembly using a hybrid strategy. We found that the predominant configuration of the mitogenome comprises two circular chromosomes. The primary structure of the mitogenome encompasses two mitochondrial chromosomes corresponding to the two major configurations, Mac1-1 and Mac1-2. The mitogenome encoded an angiosperm-typical set of 24 core genes, nine variable genes, three rRNA genes, and 15 tRNA genes. A phylogenetic analysis using the 16 shared protein-coding genes (PCG) yielded a tree consistent with the phylogeny of Lamiales species and two outgroup taxa. Mapping RNA-seq data to the coding sequences (CDS) of the PCGs revealed 507 C-to-U RNA editing sites across 31 PCGs of the mitogenome. Furthermore, one start codon (nad4L) and two stop codons (rpl10 and atp6) were identified as products of RNA editing events in the mitogenome.
是一种具有重要经济意义的药用植物。然而，其线粒体基因组的结构和序列尚未公布，线粒体基因组在进化分析和调节呼吸相关大分子合成中起着关键作用。在本研究中，采用Illumina短读长和Nanopore长读长相结合的方法对线粒体基因组进行测序，随后使用混合策略进行组装。我们发现线粒体基因组的主要构型由两个环状染色体组成。线粒体基因组的主要结构包括对应于两种主要构型Mac1-1和Mac1-2的两个线粒体染色体。该线粒体基因组编码一套被子植物典型的24个核心基因、9个可变基因、3个rRNA基因和15个tRNA基因。使用16个共享蛋白质编码基因（PCG）进行的系统发育分析产生了一棵与唇形目物种和两个外类群分类单元的系统发育一致的树。将RNA-seq数据映射到PCG的编码序列（CDS）上，发现在该线粒体基因组的31个PCG中共有507个C-to-U RNA编辑位点。此外，一个起始密码子（nad4L）和两个终止密码子（rpl10和atp6）被鉴定为该线粒体基因组中RNA编辑事件的产物。
