Bueno Cleuber Rodrigo de Souza, Buchaim Daniela Vieira, Barraviera Benedito, Ferreira Rui Seabra, Santos Paulo Sérgio da Silva, Reis Carlos Henrique Bertoni, Cini Marcelo Augusto, Kuga Milton Carlos, Rosa Geraldo Marco, Buchaim Rogerio Leone
Department of Biological Sciences, Bauru School of Dentistry (FOB), University of São Paulo (USP), Bauru, SP, Brazil.
Dentistry School, University Center of Adamantina (UNIFAI), Adamantina, SP, Brazil.
J Venom Anim Toxins Incl Trop Dis. 2024 May 27;30:e20230093. doi: 10.1590/1678-9199-JVATITD-2023-0093. eCollection 2024.
In this experimental protocol, we evaluated the immediate and delayed repair of the buccal branch of the facial nerve (BBFN) with heterologous fibrin biopolymer (HFB) as a coaptation medium and the use of photobiomodulation (PBM), performing functional and histomorphometric analysis of the BBFN and perioral muscles.
Twenty-eight rats were divided into eight groups using the BBFN bilaterally (the left nerve was used for PBM), namely: G1 - control group, right BBFN (without injury); G2 - control group, left BBFN (without injury + PBM); G3 - Denervated right BBFN (neurotmesis); G4 - Denervated left BBFN (neurotmesis + PBM); G5 - Immediate repair of right BBFN (neurotmesis + HFB); G6 - Immediate repair of left BBFN (neurotmesis + HFB + PBM); G7 - Delayed repair of right BBFN (neurotmesis + HFB); G8 - Delayed repair of left BBFN (neurotmesis + HFB + PBM). Delayed repair occurred after two weeks of denervation. All animals were sacrificed after six weeks postoperatively.
In the parameters of the BBFN, we observed inferior results in the groups with delayed repair, in relation to the groups with immediate repair, with a significant difference ( < 0.05) in the diameter of the nerve fiber, the axon, and the thickness of the myelin sheath of the group with immediate repair with PBM compared to the other experimental groups. In measuring the muscle fiber area, groups G7 (826.4 ± 69.90) and G8 (836.7 ± 96.44) were similar to G5 (882.8 ± 70.51). In the functional analysis, the G7 (4.10 ± 0.07) and G8 (4.12 ± 0.08) groups presented normal parameters.
We demonstrated that delayed repair of BBFN is possible with HFB, but with worse results compared to immediate repair, and that PBM has a positive influence on nerve regeneration results in immediate repair.
在本实验方案中，我们评估了使用异源纤维蛋白生物聚合物（HFB）作为接合介质对面神经颊支（BBFN）进行即刻和延迟修复，并使用光生物调节（PBM），同时对面神经颊支和口周肌肉进行功能及组织形态计量学分析。
将28只大鼠双侧使用BBFN（左侧神经用于PBM）分为八组，即：G1 - 对照组，右侧BBFN（未损伤）；G2 - 对照组，左侧BBFN（未损伤 + PBM）；G3 - 右侧BBFN失神经支配（神经断伤）；G4 - 左侧BBFN失神经支配（神经断伤 + PBM）；G5 - 右侧BBFN即刻修复（神经断伤 + HFB）；G6 - 左侧BBFN即刻修复（神经断伤 + HFB + PBM）；G7 - 右侧BBFN延迟修复（神经断伤 + HFB）；G8 - 左侧BBFN延迟修复（神经断伤 + HFB + PBM）。延迟修复在失神经支配两周后进行。所有动物在术后六周处死。
在BBFN的参数方面，我们观察到与即刻修复组相比，延迟修复组的结果较差，与其他实验组相比，即刻修复并使用PBM组的神经纤维直径、轴突和髓鞘厚度存在显著差异（<0.05）。在测量肌肉纤维面积时，G7组（826.4 ± 69.90）和G8组（836.7 ± 96.44）与G5组（882.8 ± 70.51）相似。在功能分析中，G7组（4.10 ± 0.07）和G8组（4.12 ± 0.08）呈现正常参数。
我们证明了使用HFB对BBFN进行延迟修复是可行的，但与即刻修复相比结果较差，并且PBM对即刻修复的神经再生结果有积极影响。
