与异源纤维蛋白生物聚合物相关的实验性神经修复后，肌肉萎缩和纤维化得到减轻。
Muscle atrophy and fibrosis are attenuated after experimental nerve repair associated with heterologous fibrin biopolymer.
作者信息
Muller Kevin Silva, Tiburcio Felipe Cantore, Ortiz Rinaldo Jose, Ferreira Junior Rui Seabra, Barraviera Benedito, de Castro E Horta José de Anchieta, Matheus Selma Maria Michelin
机构信息
Department of Structural and Functional Biology, Institute of Biosciences, São Paulo State University (Unesp), Botucatu, Brazil.
Botucatu Medical School (FMB), São Paulo State University (Unesp), Botucatu, Brazil.
出版信息
Regen Med. 2025 Apr;20(4):133-146. doi: 10.1080/17460751.2025.2507480. Epub 2025 May 23.
BACKGROUND
Neurotmesis leads to neuromuscular junction (NMJ) degeneration, muscle atrophy, and functional loss. While neurorrhaphy is standard, motor recovery is often incomplete. Heterologous fibrin biopolymer (HFB) shows potential as an adjunct, hence, we investigate HFB's late regenerative effects.
MATERIAL/METHODS: Twenty adult male Wistar rats (CEUA-FMB 1402/2021) were divided into Control (C), Denervated (D), Neurorrhaphy (N), and Neurorrhaphy + HFB (NB) groups. After 120 days, nerves and muscles were analyzed.
RESULTS
NB (1355 ± 170.4) showed more intact axons than C (927 ± 170.4, = .0026) and N (774 ± 158.2, = .0002). NMJ morphology in NB was closer to C than N, with increased nAChR alpha-1 (NB vs. N = .0428; NB vs C = .0084) and Rapsyn (NB vs. N = .0130; NB vs C = .0053) expression. Muscle integrity in NB resembled C, exhibiting less atrophy (area: C vs. N = .0002; NB vs. N = .0117; perimeter: C vs. N = .0002; NB vs. N = .0114; central nuclei: C vs. N = .0009; NB vs. N = .0110) and fibrosis (C vs. N = .0061; N vs. NB = .0326) compared to N.
CONCLUSION
HFB associated with neurorrhaphy enhanced muscle and nerve regeneration, attenuating muscle atrophy and fibrosis.
背景
神经断伤会导致神经肌肉接头（NMJ）退化、肌肉萎缩和功能丧失。虽然神经缝合术是标准治疗方法，但运动恢复往往不完全。异源纤维蛋白生物聚合物（HFB）显示出作为辅助治疗手段的潜力，因此，我们研究了HFB的后期再生作用。
材料/方法：将20只成年雄性Wistar大鼠（CEUA-FMB 1402/2021）分为对照组（C）、去神经组（D）、神经缝合组（N）和神经缝合+HFB组（NB）。120天后，对神经和肌肉进行分析。
结果
NB组（1355±170.4）的完整轴突比C组（927±170.4，P = 0.0026）和N组（774±158.2，P = 0.0002）更多。NB组的NMJ形态比N组更接近C组，nAChRα-1（NB组与N组比较，P = 0.0428；NB组与C组比较，P = 0.0084）和Rapsyn（NB组与N组比较，P = 0.0130；NB组与C组比较，P = 0.0053）表达增加。NB组的肌肉完整性与C组相似，与N组相比，萎缩程度更小（面积：C组与N组比较，P = 0.0002；NB组与N组比较，P = 0.0117；周长：C组与N组比较，P = 0.0002；NB组与N组比较，P = 0.0114；中央核：C组与N组比较，P = 0.0009；NB组与N组比较，P = 0.0110），纤维化程度更低（C组与N组比较，P = 0.0061；N组与NB组比较，P = 0.0326）。
结论
与神经缝合术联合使用的HFB可增强肌肉和神经再生，减轻肌肉萎缩和纤维化。
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