基于酸性脱氧核酶的纸基器件无扩增检测 。
Amplification-free detection of using an acidic deoxyribozyme-based paper device.
机构信息
School of Environmental Science and Technology, Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), Dalian University of Technology, Dalian, 116024, China.
Dalian POCT laboratory, Dalian, 116024, China.
出版信息
Chem Commun (Camb). 2024 Jun 27;60(53):6741-6744. doi: 10.1039/d4cc01150c.
We reported a colorimetric paper-based device by integrating the modified acid RNA-cleaving DNAzymes (MaRCD-EC1) for highly sensitive (detection limit = 10 CFU mL), and rapid (within 30 min) detection of without amplification. This device exhibited a clinical sensitivity of 100% and a specificity of 100% in identifying -associated urinary tract infections (UTIs) using the clinical urine samples.
我们报道了一种比色纸基设备，通过整合修饰的酸 RNA 切割 DNA 酶 (MaRCD-EC1) 实现了无需扩增的高度敏感（检测限=10 CFU mL）和快速（30 分钟内）检测。该设备使用临床尿液样本鉴定与相关的尿路感染 (UTI) 的临床灵敏度为 100%，特异性为 100%。
Zotero 插件