Department of Veterinary Gynaecology and Obstetrics, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry, India.
Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary Science, Mannuthy, Kerala, India.
Cryo Letters. 2024 Jul-Aug;45(4):194-211.
This comprehensive review delves into the evolving landscape of assisted reproductive technologies (ARTs) in bovine species, particularly focusing on the pivotal roles of semen additives in the cryopreservation of buffalo and cattle semen. In developing nations, where ARTs are still emerging, these techniques significantly influence bovine reproductive strategies. In contrast, developed regions have embraced them as primary approaches for dairy buffalo and cattle breeding. Semen cryopreservation, while offering advantages like extended storage and genetic propagation, also presents challenges. These include diminished sperm quality due to reactive oxygen species (ROS) production, alterations in sperm structure, and temperature fluctuations. Further, the effect of cryopreservation differs between cattle and buffaloes, with the latter exhibiting poorer semen viability and fertility due to inherent lipid composition susceptibilities. The generation and implications of ROS, especially hydrogen peroxide, contribute significantly to sperm DNA damage and functional impairments. To counteract these challenges, research has intensified on semen additives, aiming to bolster semen quality and protect against oxidative stress-induced damage. As the field advances, the review emphasizes the need for optimized cryopreservation techniques and tailored antioxidant strategies to harness the full potential of ARTs in bovine breeding programs. Doi.org/10.54680/fr24410110112.
这篇全面综述深入探讨了辅助生殖技术（ART）在牛科动物中的发展现状，特别关注精液添加剂在水牛和牛精液冷冻保存中的关键作用。在发展中国家，这些技术仍处于起步阶段，对牛科动物的繁殖策略产生了重大影响。相比之下，发达国家已经将其作为奶牛水牛和牛的主要繁殖方法。精液冷冻保存虽然具有延长储存和遗传繁殖等优势，但也带来了一些挑战。这些挑战包括由于活性氧（ROS）产生导致精子质量下降、精子结构改变以及温度波动等。此外，冷冻保存对牛和水牛的影响不同，由于内在的脂质组成易感性，后者的精液活力和生育力较差。ROS 的产生及其影响，特别是过氧化氢，对精子 DNA 损伤和功能障碍有重要影响。为了应对这些挑战，研究人员已经加强了对精液添加剂的研究，旨在提高精液质量并防止氧化应激引起的损伤。随着该领域的发展，综述强调需要优化冷冻保存技术和制定有针对性的抗氧化策略，以充分发挥辅助生殖技术在牛科动物繁殖计划中的潜力。Doi.org/10.54680/fr24410110112。
