Van der Krift T P, Leunissen J, Teerlink T, Van Heusden G P, Verkleij A J, Wirtz K W
Biochim Biophys Acta. 1985 Jan 25;812(2):387-92. doi: 10.1016/0005-2736(85)90313-x.
An antibody against the non-specific lipid transfer protein from rat liver was purified by immunoabsorbent affinity chromatography. This antibody in conjunction with protein A-colloidal gold was used to localize the transfer protein in rat liver by electron microscopy. Labeling by this immunocytochemical technique was found to be mainly restricted to the peroxisomes; low labeling was observed in the cytoplasm. Subsequent analysis of isolated peroxisomes by immunoblotting indicated that the non-specific lipid transfer protein (mol. wt. 14800) was absent from this organelle and that a protein of molecular weight 58000 was responsible for the immunological response. Immunoblotting of the membrane-free cytosol showed the presence of both proteins. It remains to be established to what extent the non-specific lipid transfer protein in the cytosol and the high-molecular weight protein in the peroxisomes are related.
通过免疫吸附亲和层析法纯化了一种针对大鼠肝脏非特异性脂质转运蛋白的抗体。该抗体与蛋白A-胶体金结合，用于通过电子显微镜在大鼠肝脏中定位转运蛋白。通过这种免疫细胞化学技术发现标记主要局限于过氧化物酶体；在细胞质中观察到低标记。随后通过免疫印迹对分离的过氧化物酶体进行分析表明，该细胞器中不存在非特异性脂质转运蛋白（分子量14800），分子量58000的一种蛋白质引起了免疫反应。对无膜胞质溶胶的免疫印迹显示这两种蛋白质都存在。细胞质中的非特异性脂质转运蛋白与过氧化物酶体中的高分子量蛋白质在何种程度上相关仍有待确定。
