来自酵母的三种转移RNA甲基转移酶的位点特异性

Site specificities of three transfer RNA methyltransferases from yeast.

作者信息

Sindhuphak T, Hellman U, Svensson I

出版信息

Biochim Biophys Acta. 1985 Jan 29;824(1):66-73. doi: 10.1016/0167-4781(85)90030-2.

Abstract

The site specificities of two distinct tRNA(m1G)methyltransferases and one tRNA(m2G)methyltransferase from yeast have been investigated by heterologous methylation and analysis of purified Escherichia coli tRNAs. The two tRNA(m1G)methyltransferases were found to be specific for sites 9 and 37, respectively. The tRNA(m2G)methyltransferase was specific for site 10. Two of the enzymes were purified by affinity chromatography on tRNA-Sepharose.

摘要

通过异源甲基化和对纯化的大肠杆菌tRNA进行分析，研究了来自酵母的两种不同的tRNA（m1G）甲基转移酶和一种tRNA（m2G）甲基转移酶的位点特异性。发现这两种tRNA（m1G）甲基转移酶分别对第9位和第37位位点具有特异性。tRNA（m2G）甲基转移酶对第10位位点具有特异性。其中两种酶通过在tRNA-琼脂糖上的亲和层析进行纯化。