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文昌鱼神经胚期细胞图谱支持脊椎动物头部中胚层出现的复杂情况。

An amphioxus neurula stage cell atlas supports a complex scenario for the emergence of vertebrate head mesoderm.

机构信息

Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology (BIST), Barcelona, Spain.

Sorbonne Université, CNRS, Biologie Intégrative des Organismes Marins, BIOM, F-66650, Banyuls-sur-Mer, France.

出版信息

Nat Commun. 2024 May 29;15(1):4550. doi: 10.1038/s41467-024-48774-4.

DOI:10.1038/s41467-024-48774-4
PMID:38811547
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11136973/
Abstract

The emergence of new structures can often be linked to the evolution of novel cell types that follows the rewiring of developmental gene regulatory subnetworks. Vertebrates are characterized by a complex body plan compared to the other chordate clades and the question remains of whether and how the emergence of vertebrate morphological innovations can be related to the appearance of new embryonic cell populations. We previously proposed, by studying mesoderm development in the cephalochordate amphioxus, a scenario for the evolution of the vertebrate head mesoderm. To further test this scenario at the cell population level, we used scRNA-seq to construct a cell atlas of the amphioxus neurula, stage at which the main mesodermal compartments are specified. Our data allowed us to validate the presence of a prechordal-plate like territory in amphioxus. Additionally, the transcriptomic profile of somite cell populations supports the homology between specific territories of amphioxus somites and vertebrate cranial/pharyngeal and lateral plate mesoderm. Finally, our work provides evidence that the appearance of the specific mesodermal structures of the vertebrate head was associated to both segregation of pre-existing cell populations, and co-option of new genes for the control of myogenesis.

摘要

新结构的出现通常可以与发育基因调控子网络的重布线所导致的新型细胞类型的进化联系起来。与其他脊索动物类群相比，脊椎动物具有复杂的身体结构，问题仍然是脊椎动物形态创新的出现是否以及如何与新的胚胎细胞群体的出现有关。我们之前通过研究头索动物文昌鱼的中胚层发育提出了一个脊椎动物头部中胚层进化的方案。为了在细胞群体水平上进一步检验这一方案，我们使用 scRNA-seq 构建了文昌鱼神经胚的细胞图谱，此时主要的中胚层区室被指定。我们的数据验证了文昌鱼中存在前脊索板样区域。此外，体节细胞群体的转录组特征支持文昌鱼体节的特定区域与脊椎动物颅面和咽旁及侧板中胚层的同源性。最后，我们的工作提供了证据表明，脊椎动物头部的特定中胚层结构的出现与已有细胞群体的分离以及新基因的获得有关，这些新基因用于控制肌发生。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec8c/11136973/4dcfb5bfd9b6/41467_2024_48774_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec8c/11136973/be94690214b3/41467_2024_48774_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec8c/11136973/8d8e647ed6dd/41467_2024_48774_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec8c/11136973/f46cf05ed5dc/41467_2024_48774_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec8c/11136973/65ce83153da9/41467_2024_48774_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec8c/11136973/c6950d386e95/41467_2024_48774_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec8c/11136973/ceb103c1bd4d/41467_2024_48774_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec8c/11136973/4dcfb5bfd9b6/41467_2024_48774_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec8c/11136973/be94690214b3/41467_2024_48774_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec8c/11136973/8d8e647ed6dd/41467_2024_48774_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec8c/11136973/f46cf05ed5dc/41467_2024_48774_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec8c/11136973/65ce83153da9/41467_2024_48774_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec8c/11136973/c6950d386e95/41467_2024_48774_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec8c/11136973/ceb103c1bd4d/41467_2024_48774_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec8c/11136973/4dcfb5bfd9b6/41467_2024_48774_Fig7_HTML.jpg

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