构建一种新型基于氨基芴的比率型近红外荧光探针，用于检测活细胞中的羧酸酯酶活性。
Construction of a novel aminofluorene-based ratiometric near-infrared fluorescence probe for detecting carboxylesterase activity in living cells.
机构信息
Key Laboratory of Green Chemistry and Technology of Ministry of Education, College of Chemistry, Sichuan University, 29, Wangjiang Road, Chengdu 610064, P. R. China.
出版信息
Anal Methods. 2024 Jun 13;16(23):3641-3645. doi: 10.1039/d4ay00501e.
Herein, we constructed a novel aminofluorene-based fluorescence probe (FEN-CE) for the detection of carboxylesterase (CE) in living cells by a ratiometric near-infrared (NIR) fluorescence signal. FEN-CE with NIR emission (650 nm) could be hydrolyzed specifically by CE and transformed to FENH with the release of the self-immolative group, which exhibited a red-shifted emission peak of 680 nm. In addition, FEN-CE showed high selectivity for CE and was successfully used in the detection of CE activity in living cells through its ratiometric NIR fluorescence signals.
在这里，我们构建了一种新型的基于氨基芴的荧光探针（FEN-CE），通过比率型近红外（NIR）荧光信号来检测活细胞中的羧酸酯酶（CE）。具有 NIR 发射（650nm）的 FEN-CE 可以被 CE 特异性水解，并在自毁基团释放后转化为 FENH，其发射峰红移至 680nm。此外，FEN-CE 对 CE 表现出高选择性，并通过其比率型 NIR 荧光信号成功用于活细胞中 CE 活性的检测。
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