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在野生型中进行的全基因组鉴定分析揭示了一些抵御……的基因。 (原文中“against”后面内容缺失)

Genome-wide identification analysis in wild-type reveals some genes defending against .

作者信息

Shen Chunxiu, Lu Qineng, Yang Di, Zhang Xueru, Huang Xinping, Li Rungen, Que Zhiqun, Chen Na

机构信息

Jiangxi Key Laboratory of Crop Growth and Development Regulation, College of Life Sciences, Resources and Environment Sciences, Yichun University, Yichun, China.

Grandomics Biosciences, Wuhan, China.

出版信息

Front Genet. 2024 May 15;15:1379784. doi: 10.3389/fgene.2024.1379784. eCollection 2024.

Abstract

exhibits strong resistance to late blight caused by but only an incomplete genome assembly based on short Illumina reads has been published. In this study, we generated the first chromosome-level draft genome for the wild-type potato species in China using Oxford Nanopore technology sequencing and Hi-C technology. The high-quality assembled genome size is 664 Mb with a scaffold N50 value of 49.17 Mb, of which 65.87% was occupied by repetitive sequences, and predominant long terminal repeats (42.51% of the entire genome). The genome of was predicted to contain 34,245 genes, of which 99.34% were functionally annotated. Moreover, 303 NBS-coding disease resistance (R) genes were predicted in the genome to investigate the potential mechanisms of resistance to late blight disease. The high-quality chromosome-level reference genome of is expected to provide potential valuable resources for intensively and effectively investigating molecular breeding and genetic research in the future.

摘要

对由……引起的晚疫病表现出很强的抗性,但目前仅发表了基于短读长Illumina测序的不完整基因组组装结果。在本研究中,我们利用牛津纳米孔技术测序和Hi-C技术,首次为中国的野生型马铃薯物种……生成了染色体水平的基因组草图。高质量组装的基因组大小为664 Mb,支架N50值为49.17 Mb,其中65.87%被重复序列占据,主要是长末端重复序列(占整个基因组的42.51%)。预计……的基因组包含34245个基因,其中99.34%具有功能注释。此外,在……基因组中预测了303个编码NBS的抗病(R)基因,以研究其对晚疫病抗性的潜在机制。预计高质量的染色体水平参考基因组……将为未来深入有效地开展分子育种和遗传研究提供潜在的宝贵资源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ec1/11134371/994d1d6ba8ab/fgene-15-1379784-g001.jpg

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