The Histological, Effectoromic, and Transcriptomic Analyses of Reveal an Upregulation of Multiple Genes Suppressing Infection.

Utilization of disease resistance components from wild potatoes is a promising and sustainable approach to control blight. Here, we combined avirulence () genes screen with RNA-seq analysis to discover the potential mechanism of resistance in Mexican wild potato species, . Histological characterization displayed that hyphal expansion was significantly restricted in epidermal cells and mesophyll cell death was predominant, indicating that a typical defense response was initiated in . Inoculation of with diverse isolates showed distinct resistance patterns, suggesting that has complex genetic resistance to most of the prevalent races of in northwestern China. Further analysis by gene screens and comparative transcriptomic profiling revealed the presence and upregulation of multiple plant genes corresponding to biotic stresses. Six alleles of , , , , , and were detected, and over 60% of the 112 detected NLR proteins were significantly induced in . On the contrary, despite the expression of the , , and alleles, fewer NLR proteins were expressed in susceptible . Thus, the enriched genes in make it an ideal genetic resource for the discovery and deployment of resistance genes for potato breeding.