State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling 712100, China.
College of Plant Protection, Northwest A&F University, Yangling 712100, China.
Int J Mol Sci. 2020 May 1;21(9):3211. doi: 10.3390/ijms21093211.
Utilization of disease resistance components from wild potatoes is a promising and sustainable approach to control blight. Here, we combined avirulence () genes screen with RNA-seq analysis to discover the potential mechanism of resistance in Mexican wild potato species, . Histological characterization displayed that hyphal expansion was significantly restricted in epidermal cells and mesophyll cell death was predominant, indicating that a typical defense response was initiated in . Inoculation of with diverse isolates showed distinct resistance patterns, suggesting that has complex genetic resistance to most of the prevalent races of in northwestern China. Further analysis by gene screens and comparative transcriptomic profiling revealed the presence and upregulation of multiple plant genes corresponding to biotic stresses. Six alleles of , , , , , and were detected, and over 60% of the 112 detected NLR proteins were significantly induced in . On the contrary, despite the expression of the , , and alleles, fewer NLR proteins were expressed in susceptible . Thus, the enriched genes in make it an ideal genetic resource for the discovery and deployment of resistance genes for potato breeding.
利用野生马铃薯的抗病成分是控制晚疫病的一种有前途和可持续的方法。在这里，我们结合无毒基因筛选和 RNA-seq 分析，发现了墨西哥野生马铃薯种（）潜在的抗性机制。组织学特征表明，菌丝在表皮细胞中的扩展受到显著限制，而叶肉细胞死亡占主导地位，表明在中启动了典型的防御反应。用不同的分离物接种表明具有明显的抗性模式，这表明对中国西北地区大多数流行的晚疫病菌具有复杂的遗传抗性。通过基因筛选和比较转录组分析进一步发现，存在并上调了多种与生物胁迫相关的植物抗病基因。检测到 6 个的等位基因，、、、、和，在中检测到超过 112 个 NLR 蛋白中的 60%以上显著诱导。相反，尽管表达了、和等位基因，但在感病中表达的 NLR 蛋白较少。因此，中丰富的抗病基因使其成为发现和部署马铃薯抗性基因进行育种的理想遗传资源。
