Urology Department , Mustafa Kemal University School of Medicine, Turkey.
Biochemistry Department , Mustafa Kemal University School of Medicine, Turkey.
Urol J. 2020 May 16;17(3):294-300. doi: 10.22037/uj.v0i0.4918.
To evaluate the short-term use of colchicine on preventing ischemia-reperfusion injury after surgery in an experimental animal model.
A total of 40 rats were divided into five groups (n = 8). Sham (Sh), ischemia-reperfusion (I/R), I/R and colchicine-treated for once per-operatively (I/Rc1), I/R and colchicine-treated for 5 days postoperatively (I/Rc5), and I/R and placebo given for 5 days (I/Rp) groups. Testicular torsion was created by rotating the testicle 720o in clockwise direction and held for 3 hours. In group I/Rc1 30 minutes before detorsion, p.o. 1 mg/kg mL infusion of colchicine was given only once. In group I/Rc5, colchicine continued p.o. once daily for five days. Tissue malonyldialdehite (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) were measured for evaluating the oxidative stress. Apoptosis levels shown with Caspase-3 staining and mean seminiferous tubular diameter (MSTD), germinal epithelial cell thickness (GECT), and mean testicular biopsy score (MTBS) were used to evaluate the germ cell damage.
Decreased protein MDA levels therewithal increased SOD, CAT and GPx levels achieved in I/Rc5 group when compared to I/R group and did not differ from the I/Rp group (p<0.05). MSTD, GECT, and JS were better in I/Rc5 than I/Rp which showed the natural course of I/R damage in testis (p<0.005). Caspase 3 positivity, as an apoptosis indicator, were significantly lower (p<0.05) in I/Rc5 group in comparison with I/R, I/Rc1, and I/Rp groups.
The usage of colchicine as a complementary treatment after definitive surgery reduce early-onset ischemia-reperfusion damage and diminishes apoptosis.
在实验动物模型中，评估秋水仙碱短期使用对预防手术后缺血再灌注损伤的作用。
将 40 只大鼠分为五组（n = 8）：假手术组（Sh）、缺血再灌注组（I/R）、单次围手术期秋水仙碱治疗组（I/Rc1）、术后连续 5 天秋水仙碱治疗组（I/Rc5）和安慰剂组（I/Rp）。通过顺时针旋转睾丸 720o 造成睾丸扭转，维持 3 小时。在 I/Rc1 组中，于松解扭转前 30 分钟，给予 1mg/kg 秋水仙碱口服溶液 1 次。在 I/Rc5 组中，给予秋水仙碱口服，每天 1 次，连续 5 天。通过测量组织丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）和过氧化氢酶（CAT）的水平来评估氧化应激。通过 Caspase-3 染色评估凋亡水平，通过平均生精小管直径（MSTD）、生精上皮细胞厚度（GECT）和平均睾丸活检评分（MTBS）评估生精细胞损伤。
与 I/R 组相比，I/Rc5 组 MDA 蛋白水平降低，SOD、CAT 和 GPx 水平升高，且与 I/Rp 组无差异（p<0.05）。与 I/Rp 组相比，I/Rc5 组的 MSTD、GECT 和 JS 更好，表明睾丸的 I/R 损伤具有自然病程（p<0.005）。作为凋亡指标的 Caspase 3 阳性率在 I/Rc5 组明显低于 I/R、I/Rc1 和 I/Rp 组（p<0.05）。
在确定性手术后使用秋水仙碱作为辅助治疗可减轻早期缺血再灌注损伤并减少凋亡。
