子痫前期大鼠耳蜗病变：维生素 D 和硫酸镁的保护作用。
Cochlear pathology in preeclamptic rats: protective effects of vitamin D and magnesium sulfate.
机构信息
Department of Otorhinolaryngology, Antalya Training and Research Hospital, University of Health Science, Antalya, Turkiye.
Department of Drug Science and Technology, University of Turin, Turin, Italy.
出版信息
Turk J Med Sci. 2023 Oct 12;53(6):1614-1620. doi: 10.55730/1300-0144.5730. eCollection 2023.
BACKGROUND/AIM: This study investigated the possible degeneration in cochlear morphology induced by preeclampsia (PE) and the therapeutic/preventive effect of vitamin D (Vit D) and magnesium sulfate (MgSO) used separately and together on feto-maternal outcomes.
MATERIALS AND METHODS
We created PE in rats using a reduced uterine perfusion pressure (RUPP) animal model and recorded blood pressure (BP), embryonic survival (ES), and embryonic weight (EW) and evaluated cochlear morphology by electron microscopy.
RESULTS
The PE group had elevated BP, a decreased number and weight of live pups, and significant degeneration in the cochlea compared to the sham group. In the PEV group, we observed significant beneficial effects of Vit D supplementation at 14.5 and 19.5 dpc in terms of BP (p < 0.05), EW (p < 0.001), and cochlear degeneration compared to the PE group. In the PEM group, BP (p < 0.05) and cochlear degeneration nearly reached the level found in the sham group. However, although the EW was statistically different in the PE group, it did not reach sham group levels. We also observed that BP returned to sham level (p < 0.01) and noticed significant increases in the EW (p < 0.0001) and ES (p = 0.017) in the PEMV group compared to the PE group. According to the scanning electron microscope results, combined administration of VitD and MgSO is more effective than separate administration in improving cochlear degeneration induced by PE.
CONCLUSION
The administration of Vit D and MgSO during pregnancy has beneficial effects on PE pathology and may play a significant role in preventing PE-related complications, including cochlear degeneration.
背景/目的：本研究旨在探讨子痫前期（PE）可能引起的耳蜗形态学退变，以及维生素 D（Vit D）和硫酸镁（MgSO）单独和联合应用于母婴结局的治疗/预防效果。
材料和方法
我们使用子宫灌注压降低（RUPP）动物模型在大鼠中创建 PE，并记录血压（BP）、胚胎存活率（ES）和胚胎体重（EW），并通过电子显微镜评估耳蜗形态。
结果
PE 组的 BP 升高，活仔数和体重减少，与假手术组相比，耳蜗明显退变。在 PEV 组中，我们观察到在 14.5 和 19.5 dpc 时补充 Vit D 对 BP（p<0.05）、EW（p<0.001）和耳蜗退变有显著的有益影响，与 PE 组相比。在 PEM 组中，BP（p<0.05）和耳蜗退变几乎达到假手术组水平。然而，尽管 PE 组的 EW 存在统计学差异，但未达到假手术组水平。我们还观察到 BP 恢复到假手术水平（p<0.01），并且在 PEMV 组中 EW（p<0.0001）和 ES（p=0.017）显著增加，与 PE 组相比。根据扫描电子显微镜结果，VitD 和 MgSO 的联合给药比单独给药在改善 PE 引起的耳蜗退变方面更有效。
结论
在妊娠期间给予 Vit D 和 MgSO 对 PE 病理有有益影响，并可能在预防与 PE 相关的并发症（包括耳蜗退变）方面发挥重要作用。
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