Yildirim E, Macun H C, Cinar M, Yiğit A A, Ustüner I, Aydos T R, Aktuna Z
Clin Exp Obstet Gynecol. 2014;41(2):169-73.
This study was undertaken to evaluate the effects of magnesium sulfate (MgSO4) on the contractile activity of the uterus in a pregnant rat model of preeclampsia induced by N-nitro-arginine methyl ester (L-NAME).
Twenty-eight, 160-220 gram, three to four month old female Sprague-Dawley rats were used in this study. After conception was confirmed by vaginal smears on the first day of pregnancy, the animals were allocated into four groups according to the chemicals fed in their drinking water as control (nothing administered), L-NAME (50 mg/kg L-NAME), MgSO4 (600 mg/kg MgSO4), and MgSO4 + L-NAME group (600 mg/kg MgSO4 + 50 mg/kg L-NAME). The pregnant uterus strips were isolated on the 19th day and the contractile activity of uterus was examined by applying 0, 0.1, 0.2, 0.4, 0.8, and 2.5 mIU/ml oxytocin to each group and responses are recorded accordingly.
There were no statistically significant differences regarding fetal parameters and peak amplitudes of the oxytocin stimulated pregnant rat myometrial strips among groups. In L-NAME group at 0 and 0.1 mIU/ml oxytocin, the contraction frequency in a ten-min period was statistically lower than the control group (Z = -2.850, p = 0.004; Z = -2.902, p = 0.004, respectively). In MgSO4 group only at 0 mIU/ml oxytocin, the frequencies of the contractions in ten-min period were statistically lower than the control group (Z = -2.973,p = 0.003). In L-NAME + MgSO4 group at 0, 0.1 and 0.2 mIU/ml oxytocin concentrations the frequencies of the contractions in ten-min period were statistically lower than the control group (Z = - 4.018, p = 0.000; Z = -3.237, p = 0.001; Z = -2.902, p = 0.004, respectively). In L-NAME + MgSO4 given group at each oxytocin concentrations, the frequencies of the contractions in ten-min period were lower but not statistically different than the L-NAME group.
MgSO4 has no significant effect on the amplitude of spontaneous or oxytocin induced myometrial contractions, but decreased the frequency of spontaneous contractions. At each doses of oxytocin, MgSO4 has no significant effect on the frequency of contraction in a pregnant rat model of preeclampsia induced by L-NAME.
本研究旨在评估硫酸镁（MgSO4）对N-硝基-精氨酸甲酯（L-NAME）诱导的子痫前期孕鼠模型子宫收缩活性的影响。
本研究使用了28只体重160 - 220克、3至4月龄的雌性Sprague-Dawley大鼠。在妊娠第一天通过阴道涂片确认受孕后，根据其饮用水中添加的化学物质将动物分为四组：对照组（未给予任何物质）、L-NAME组（50毫克/千克L-NAME）、MgSO4组（600毫克/千克MgSO4）和MgSO4 + L-NAME组（600毫克/千克MgSO4 + 50毫克/千克L-NAME）。在第19天分离出妊娠子宫条，对每组施加0、0.1、0.2、0.4、0.8和2.5毫国际单位/毫升的缩宫素，检测子宫的收缩活性，并相应记录反应。
各组之间在胎儿参数和缩宫素刺激的孕鼠子宫肌条的峰值幅度方面无统计学显著差异。在L-NAME组中，当缩宫素浓度为0和0.1毫国际单位/毫升时，十分钟内的收缩频率在统计学上低于对照组（Z = -2.850，p = 0.004；Z = -2.902，p = 0.004）。在MgSO4组中，仅当缩宫素浓度为0毫国际单位/毫升时，十分钟内的收缩频率在统计学上低于对照组（Z = -2.973，p = 0.003）。在L-NAME + MgSO4组中，当缩宫素浓度为0、0.1和0.2毫国际单位/毫升时，十分钟内的收缩频率在统计学上低于对照组（分别为Z = - 4.018，p = 0.000；Z = -3.237，p = 0.001；Z = -2.902，p = 0.004）。在给予L-NAME + MgSO4的组中，在每个缩宫素浓度下，十分钟内的收缩频率均低于L-NAME组，但无统计学差异。
MgSO4对自发或缩宫素诱导的子宫肌层收缩幅度无显著影响，但可降低自发收缩频率。在L-NAME诱导的子痫前期孕鼠模型中，在每个缩宫素剂量下，MgSO4对收缩频率均无显著影响。
