Kapperud G, Namork E, Skarpeid H J
Infect Immun. 1985 Feb;47(2):561-6. doi: 10.1128/iai.47.2.561-566.1985.
When cultivated at 37 degrees C in static broth, human clinical isolates of Yersinia enterocolitica (serogroups O:3, O:8, and O:9) and Yersinia pseudotuberculosis (serogroup O:III) produced numerous nonflagellar surface appendages, which appeared as a lawn of fine fibrillae, each having a diameter of 1.5 to 2.0 nm and a length of 50 to 70 nm. Cultivation at 22 degrees C resulted in complete disappearance of the fibrillae. The phenotypic expression of these appendages was correlated with the presence of the 40- to 48-megadalton virulence plasmid and was strongly affected by the growth medium. Evidence is presented which suggests that these plasmid-mediated, temperature-inducible surface fibrillae are responsible for autoagglutination and are related to production of one prominent, Sarkosyl-insoluble polypeptide of ca. 180 kilodaltons in the bacterial outer membrane.
当在37摄氏度的静止肉汤中培养时，人源小肠结肠炎耶尔森菌（血清型O:3、O:8和O:9）和假结核耶尔森菌（血清型O:III）的临床分离株产生了大量非鞭毛表面附属物，这些附属物看起来像一层纤细的纤丝，每根纤丝的直径为1.5至2.0纳米，长度为50至70纳米。在22摄氏度下培养导致纤丝完全消失。这些附属物的表型表达与40至48兆道尔顿毒力质粒的存在相关，并受到生长培养基的强烈影响。有证据表明，这些由质粒介导、温度诱导的表面纤丝负责自身凝集，并且与细菌外膜中一种约180千道尔顿的突出的、不溶于 Sarkosyl 的多肽的产生有关。
