Feng Yayu, Liu Yunfei, Sun Yue, Zhang Meijing, Zhang Pengfei, Zhao Ronghui, Deng Kuilin
Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Materials Science, Hebei University, Baoding 071002, China.
Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Materials Science, Hebei University, Baoding 071002, China; Department of Clinical Pharmacy, Affiliated Hospital of Hebei University, Baoding 071002, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2024 Oct 5;318:124502. doi: 10.1016/j.saa.2024.124502. Epub 2024 May 24.
In this study, four polyureas with triazine moiety (PUAs) were successfully synthesized through the polymerization of triazine-containing diamine and diisocyanate. The intramolecular aggregation of triazine rings and urea groups along the macromolecular backbone gives PUAs a significant polymerization-induced emission (PIE). Among the four PUAs, PUA-LP shows a significant fluorescent emission at 450 nm, compared to non/weak fluorescent 2,4-diamino-6-phenyl-1,3,5-triazine and L-Lysine diisocyanate ethyl ester monomers. Additionally, the external factors such as solution concentration, excitation wavelength, and precipitants also play a crucial role in the fluorescence of PUAs. As expected, PUA-LP can selectively recognize and detect Fe/Fe ions even in the presence of 12 other metal ions and 10 anions. The limit of detection of PUA-LP to FeFe is as low as 1.02 μM (0.06 mg/L) and 0.86 μM (0.05 mg/L), respectively, and far below 0.3 mg/L of the allowable national standard for drinking water by WHO. Furthermore, the quenching mechanism of FeFe to PUA-LP is attributed to static quenching caused by the coordination of Fe/Fe ions with a coordination ratio of 2:1. Based on PIE, the fluorescent PUA-LP was made into an observable and portable testing paper for detecting FeFe ions. Finally, we measured the recovery rate of the actual tap water samples and compared the performance of PIE-active PUA-LP with the other reported fluorescent probes to Fe/Fe ions.
在本研究中，通过含三嗪二胺与二异氰酸酯的聚合反应成功合成了四种含三嗪基团的聚脲（PUA）。三嗪环和脲基沿大分子主链的分子内聚集赋予了PUA显著的聚合诱导发光（PIE）特性。在这四种PUA中，与无荧光/弱荧光的2,4 - 二氨基 - 6 - 苯基 - 1,3,5 - 三嗪和L - 赖氨酸二异氰酸乙酯单体相比，PUA - LP在450 nm处显示出显著的荧光发射。此外，溶液浓度、激发波长和沉淀剂等外部因素对PUA的荧光也起着关键作用。正如预期的那样，即使在存在12种其他金属离子和10种阴离子的情况下，PUA - LP仍能选择性地识别和检测Fe²⁺/Fe³⁺离子。PUA - LP对Fe²⁺/Fe³⁺的检测限分别低至1.02 μM（0.06 mg/L）和0.86 μM（0.05 mg/L），远低于世界卫生组织规定的0.3 mg/L的饮用水国家标准。此外，Fe²⁺/Fe³⁺对PUA - LP的猝灭机制归因于Fe²⁺/Fe³⁺离子以2:1的配位比配位引起的静态猝灭。基于PIE，将荧光PUA - LP制成了用于检测Fe²⁺/Fe³⁺离子的可观察且便携的测试纸。最后，我们测量了实际自来水样品的回收率，并将具有PIE活性的PUA - LP与其他报道的用于检测Fe²⁺/Fe³⁺离子的荧光探针的性能进行了比较。
