Kosikov K V, Medvedeva A A
Mikrobiologiia. 1979 Sep-Oct;48(5):887-91.
Crossing the cells of Saccharomyces (diploid) and Schizosaccharomyces (haploid) cells as well as using genetic markers (adenine dependent mutants) and selective media produced remote hybrids. The hybrid cultures possessed the intermediate type of inheritance in a number of properties. Along with budding typical of Saccharomyces, partitions characteristic of Schizosaccharomyces were encountered. The shape and size of cells and some physiological properties were found to vary in the course of vegetative growth (no spores were formed by the hybrid cultures). Conditions are discussed which favour remote hybridization of yeast cultures.
将酿酒酵母(二倍体)细胞与裂殖酵母(单倍体)细胞杂交,并使用遗传标记(腺嘌呤依赖型突变体)和选择性培养基,获得了远缘杂种。杂种培养物在许多特性上具有中间型遗传。除了酿酒酵母典型的出芽方式外,还观察到了裂殖酵母特有的分隔现象。发现细胞的形状和大小以及一些生理特性在营养生长过程中会发生变化(杂种培养物不形成孢子)。文中讨论了有利于酵母培养物远缘杂交的条件。