Graves H C, Sensabaugh G F, Blake E T
N Engl J Med. 1985 Feb 7;312(6):338-43. doi: 10.1056/NEJM198502073120603.
Identification of semen in vaginal fluid may provide documentation of sexual contact in alleged victims of rape. We describe an enzyme-linked immunosorbent assay for a semen glycoprotein of prostatic origin, designated p30. This test detects as little as 3 ng of the p30 antigen per milliliter in various body fluids. Semen from normal and vasectomized men contains high levels of p30 (mean, 1.55 mg per milliliter of seminal plasma), and urine from men contains low levels (mean, 260 ng per milliliter). However, the antigen cannot be detected in body fluids from women, including vaginal fluid and urine, suggesting that p30 may be a male-specific antigen. The p30 antigen was detectable in vaginal fluid for a mean period of 27 hours after coitus, as compared with 14 hours for prostatic acid phosphatase. Of 27 vaginal fluid samples from women who were allegedly raped in which the acid phosphatase test was negative, 7 (26 per cent) were unequivocally positive for p30 by our assay. We conclude that the assay for p30 offers a more sensitive and specific method of semen detection in rape investigation than the enzyme assay for prostatic acid phosphatase.
在涉嫌强奸案的受害者阴道分泌物中鉴定出精液，可为性接触提供证据。我们描述了一种针对前列腺来源的精液糖蛋白（命名为p30）的酶联免疫吸附测定法。该检测方法能在各种体液中检测到低至每毫升3纳克的p30抗原。正常男性和输精管切除术后男性的精液中p30含量很高（平均每毫升精浆含1.55毫克），男性尿液中含量较低（平均每毫升260纳克）。然而，在女性的体液中，包括阴道分泌物和尿液中，无法检测到该抗原，这表明p30可能是一种男性特异性抗原。性交后，阴道分泌物中p30抗原平均可检测到27小时，而前列腺酸性磷酸酶为14小时。在27份来自涉嫌强奸女性的阴道分泌物样本中，酸性磷酸酶检测呈阴性，其中7份（26%）通过我们的检测p30呈明确阳性。我们得出结论，在强奸案调查中，p30检测比前列腺酸性磷酸酶酶检测提供了一种更敏感、更特异的精液检测方法。
