在存在一系列可能污染物的情况下，通过酶联免疫吸附测定法检测P30和19-羟基前列腺素F1/F2。
The detection by enzyme linked immunosorbent assay of P30 and 19-OH prostaglandin F1/F2, in the presence of a range of possible contaminants.
作者信息
Sutton J G, Bosley C, Rands A
机构信息
Forensic Science Service, London Laboratory, United Kingdom.
出版信息
Sci Justice. 1998 Jul-Sep;38(3):157-64. doi: 10.1016/S1355-0306(98)72100-8.
The potential of specific human semen markers, P30 and 19-OH prostaglandin F1 alpha/F2 alpha (PG F), ELISAs were compared in a blind trial. The PG F assay was shown to be at least ten times more sensitive than the P30 technique although cross reactivity problems with spermicidal creams and other possible domestic contaminants were identified. A simple method for the removal of such contaminants using a phenyl boronate column (PBA) was evaluated in an extensive blind trial.
在一项盲法试验中，对特定人类精液标志物P30和19-羟基前列腺素F1α/F2α（PG F）酶联免疫吸附测定（ELISA）的潜力进行了比较。结果表明，PG F检测的灵敏度至少比P30技术高十倍，不过发现了其与杀精乳膏及其他可能的家庭污染物存在交叉反应问题。在一项广泛的盲法试验中，评估了一种使用苯基硼酸酯柱（PBA）去除此类污染物的简单方法。
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