Meng Han, Nan Fu-Yao, Kou Na, Hong Qin-Yan, Lv Ming-Sheng, Li Ju-Bo, Zhang Bao-Jie, Zou Hang, Li Lei, Wang Hong-Wu
Respiratory Disease Center, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100029, China.
The First Clinical Medical College, Beijing University of Chinese Medicine, Beijing 100700, China.
World J Gastrointest Surg. 2024 May 27;16(5):1385-1394. doi: 10.4240/wjgs.v16.i5.1385.
Previous studies have validated the efficacy of both magnetic compression and surgical techniques in creating rabbit tracheoesophageal fistula (TEF) models. Magnetic compression achieves a 100% success rate but requires more time, while surgery, though less frequently successful, offers rapid model establishment and technical maturity in larger animal models.
To determine the optimal approach for rabbit disease modeling and refine the process.
TEF models were created in 12 rabbits using both the modified magnetic compression technique and surgery. Comparisons of the time to model establishment, success rate, food and water intake, weight changes, activity levels, bronchoscopy findings, white blood cell counts, and biopsies were performed. In response to the failures encountered during modified magnetic compression modeling, we increased the sample size to 15 rabbit models and assessed the repeatability and stability of the models, comparing them with the original magnetic compression technique.
The modified magnetic compression technique achieved a 66.7% success rate, whereas the success rate of the surgery technique was 33.3%. Surviving surgical rabbits might not meet subsequent experimental requirements due to TEF-related inflammation. In the modified magnetic compression group, one rabbit died, possibly due to magnet corrosion, and another died from tracheal magnet obstruction. Similar events occurred during the second round of modified magnetic compression modeling, with one rabbit possibly succumbing to aggravated lung infection. The operation time of the first round of modified magnetic compression was 3.2 ± 0.6 min, which was significantly reduced to 2.1 ± 0.4 min in the second round, compared to both the first round and that of the original technique.
The modified magnetic compression technique exhibits lower stress responses, a simple procedure, a high success rate, and lower modeling costs, making it a more appropriate choice for constructing TEF models in rabbits.
先前的研究已验证了磁压缩和手术技术在创建兔气管食管瘘（TEF）模型方面的有效性。磁压缩成功率达100%，但耗时更长；而手术虽然成功率较低，但能快速建立模型且在大型动物模型中技术成熟。
确定兔疾病建模的最佳方法并优化流程。
使用改良磁压缩技术和手术在12只兔中创建TEF模型。对模型建立时间、成功率、食物和水摄入量、体重变化、活动水平、支气管镜检查结果、白细胞计数及活检进行比较。针对改良磁压缩建模过程中遇到的失败情况，将样本量增加至15只兔模型，并评估模型的可重复性和稳定性，与原始磁压缩技术进行比较。
改良磁压缩技术成功率为66.7%，而手术技术成功率为33.3%。存活的手术兔可能因TEF相关炎症而不符合后续实验要求。在改良磁压缩组中，一只兔死亡，可能是由于磁体腐蚀，另一只死于气管磁体阻塞。第二轮改良磁压缩建模期间也发生了类似事件，一只兔可能死于加重的肺部感染。第一轮改良磁压缩的手术时间为3.2±0.6分钟，与第一轮及原始技术相比，第二轮显著缩短至2.1±0.4分钟。
改良磁压缩技术应激反应较低、操作简单、成功率高且建模成本低，是构建兔TEF模型更合适的选择。
