Heath Joseph R, Fromuth Daniel P, Dembowski Jill A
Department of Biological Sciences, Duquesne University, Pittsburgh, Pennsylvania, United States.
MicroPubl Biol. 2024 May 15;2024. doi: 10.17912/micropub.biology.001171. eCollection 2024.
Proteomic analysis of viral and cellular proteins that copurify with the herpes simplex virus type-1 (HSV-1) genome revealed that the cellular Integrator complex associates with viral DNA throughout infection. The Integrator complex plays a key role in the regulation of transcription of cellular coding and non-coding RNAs. We therefore predicted that it may regulate transcription of viral genes. Here, we demonstrate that knockdown of the Integrator complex subunit, Ints3, has minimal effect on HSV-1 infection. Despite reducing viral yield during low multiplicity infection, Ints3 knockdown had no effect on viral DNA replication, mRNA expression, or yield during high multiplicity infection.
对与1型单纯疱疹病毒（HSV-1）基因组共纯化的病毒和细胞蛋白进行蛋白质组学分析发现，在整个感染过程中，细胞整合酶复合物与病毒DNA相关联。整合酶复合物在细胞编码和非编码RNA转录调控中起关键作用。因此，我们推测它可能调控病毒基因的转录。在此，我们证明敲低整合酶复合物亚基Ints3对HSV-1感染影响极小。尽管在低 multiplicity 感染期间降低了病毒产量，但Ints3敲低对高 multiplicity 感染期间的病毒DNA复制、mRNA表达或产量没有影响。
