Yu Xiaoli, Tu Qichao, Liu Jihua, Peng Yisheng, Wang Cheng, Xiao Fanshu, Lian Yingli, Yang Xueqin, Hu Ruiwen, Yu Huang, Qian Lu, Wu Daoming, He Ziying, Shu Longfei, He Qiang, Tian Yun, Wang Faming, Wang Shanquan, Wu Bo, Huang Zhijian, He Jianguo, Yan Qingyun, He Zhili
State Key Laboratory for Biocontrol, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Environmental Science and Engineering, Environmental Microbiomics Research Center Sun Yat-sen University Guangzhou China.
Institute of Marine Science and Technology Shandong University Qingdao China.
mLife. 2023 Sep 3;2(3):253-266. doi: 10.1002/mlf2.12077. eCollection 2023 Sep.
Mangrove reforestation with introduced species has been an important strategy to restore mangrove ecosystem functioning. However, how such activities affect microbially driven methane (CH), nitrogen (N), and sulfur (S) cycling of rhizosphere microbiomes remains unclear. To understand the effect of environmental selection and the evolutionary process on microbially driven biogeochemical cycles in native and introduced mangrove rhizospheres, we analyzed key genomic and functional profiles of rhizosphere microbiomes from native and introduced mangrove species by metagenome sequencing technologies. Compared with the native mangrove (, KO), the introduced mangrove (, SA) rhizosphere microbiome had significantly ( < 0.05) higher average genome size (AGS) (5.8 vs. 5.5 Mb), average 16S ribosomal RNA gene copy number (3.5 vs. 3.1), relative abundances of mobile genetic elements, and functional diversity in terms of the Shannon index (7.88 vs. 7.84) but lower functional potentials involved in CH cycling (e.g., and ), N fixation (), and inorganic S cycling (, , , , , and ). Similar results were also observed from the recovered Proteobacterial metagenome-assembled genomes with a higher AGS and distinct functions in the introduced mangrove rhizosphere. Additionally, salinity and ammonium were identified as the main environmental drivers of functional profiles of mangrove rhizosphere microbiomes through deterministic processes. This study advances our understanding of microbially mediated biogeochemical cycling of CH, N, and S in the mangrove rhizosphere and provides novel insights into the influence of environmental selection and evolutionary processes on ecosystem functions, which has important implications for future mangrove reforestation.
引入物种进行红树林重新造林一直是恢复红树林生态系统功能的重要策略。然而，此类活动如何影响根际微生物群落中由微生物驱动的甲烷（CH）、氮（N）和硫（S）循环仍不清楚。为了了解环境选择和进化过程对原生和引入红树林根际中由微生物驱动的生物地球化学循环的影响，我们通过宏基因组测序技术分析了原生和引入红树林物种根际微生物群落的关键基因组和功能特征。与原生红树林（，KO）相比，引入红树林（，SA）根际微生物群落的平均基因组大小（AGS）显著更高（<0.05）（5.8对5.5 Mb）、平均16S核糖体RNA基因拷贝数更高（3.5对3.1）、移动遗传元件的相对丰度更高，并且在香农指数方面功能多样性更高（7.88对7.84），但参与CH循环（例如，和）、固氮（）和无机S循环（，，，，，和）的功能潜力较低。从回收的变形菌门宏基因组组装基因组中也观察到了类似结果，其在引入红树林根际中具有更高的AGS和不同的功能。此外，通过确定性过程确定盐度和铵是红树林根际微生物群落功能特征的主要环境驱动因素。这项研究推进了我们对红树林根际中CH、N和S的微生物介导生物地球化学循环的理解，并为环境选择和进化过程对生态系统功能的影响提供了新的见解，这对未来的红树林重新造林具有重要意义。
